Acetylcholinesterase (ACHE) Human shRNA Plasmid Kit (Locus ID 43)

Specifications

Product Data
Product Name	Acetylcholinesterase (ACHE) Human shRNA Plasmid Kit (Locus ID 43)
Locus ID	43
UniProt ID	P22303
Synonyms	ACEE; ARACHE; N-ACHE; YT
Vector	pRS
Format	Retroviral plasmids
Kit Components	ACHE - Human, 4 unique 29mer shRNA constructs in retroviral untagged vector(Gene ID = 43). 5µg purified plasmid DNA per construct 29-mer scrambled shRNA cassette in pRS Vector, TR30012, included for free.
RefSeq	NM_000665, NM_001282449, NM_001302621, NM_001302622, NM_015831, NM_000665.1, NM_000665.2, NM_000665.3, NM_000665.4, NM_015831.1, NM_015831.2, NM_001282449.1, NM_001302622.1, NM_001302621.1, BC094752, BC105060, BC105060.1, BC001541, BC026315, BC036813, BC105062, BC143469, NM_001367919, NM_001367917, NM_001367918, NR_160407, NM_001367915, NR_160408, NM_001302622.2, NM_001282449.2, NM_001302621.3, NM_000665.5
Summary	Acetylcholinesterase hydrolyzes the neurotransmitter, acetylcholine at neuromuscular junctions and brain cholinergic synapses, and thus terminates signal transmission. It is also found on the red blood cell membranes, where it constitutes the Yt blood group antigen. Acetylcholinesterase exists in multiple molecular forms which possess similar catalytic properties, but differ in their oligomeric assembly and mode of cell attachment to the cell surface. It is encoded by the single ACHE gene, and the structural diversity in the gene products arises from alternative mRNA splicing, and post-translational associations of catalytic and structural subunits. The major form of acetylcholinesterase found in brain, muscle and other tissues is the hydrophilic species, which forms disulfide-linked oligomers with collagenous, or lipid-containing structural subunits. The other, alternatively spliced form, expressed primarily in the erythroid tissues, differs at the C-terminal end, and contains a cleavable hydrophobic peptide with a GPI-anchor site. It associates with the membranes through the phosphoinositide (PI) moieties added post-translationally. AChE activity may constitute a sensitive biomarker of RBC ageing in vivo, and thus, may be of aid in understanding the effects of transfusion[provided by RefSeq, Sep 2019]
shRNA Design	These shRNA constructs were designed against multiple splice variants at this gene locus. To be certain that your variant of interest is targeted, please contact techsupport@origene.com. If you need a special design or shRNA sequence, please utilize our custom shRNA service.
Performance Guaranteed	OriGene guarantees that the sequences in the shRNA expression cassettes are verified to correspond to the target gene with 100% identity. One of the four constructs at minimum are guaranteed to produce 70% or more gene expression knock-down provided a minimum transfection efficiency of 80% is achieved. Western Blot data is recommended over qPCR to evaluate the silencing effect of the shRNA constructs 72 hrs post transfection. To properly assess knockdown, the gene expression level from the included scramble control vector must be used in comparison with the target-specific shRNA transfected samples. For non-conforming shRNA, requests for replacement product must be made within ninety (90) days from the date of delivery of the shRNA kit. To arrange for a free replacement with newly designed constructs, please contact Technical Services at techsupport@origene.com. Please provide your data indicating the transfection efficiency and measurement of gene expression knockdown compared to the scrambled shRNA control (Western Blot data preferred).

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.