POLR2J2 Human shRNA Plasmid Kit (Locus ID 246721)

Specifications

Product Data
Product Name	POLR2J2 Human shRNA Plasmid Kit (Locus ID 246721)
Locus ID	246721
UniProt ID	Q9GZM3
Synonyms	DNA directed RNA polymerase II polypeptide J-related; HRPB11B; MGC54043; MGC105050; polymerase (RNA) II (DNA directed) polypeptide J2; RPB11, HRPB11B, MGC54043, MGC105050; RPB11b1
Vector	pRS
Format	Retroviral plasmids
Kit Components	POLR2J2 - Human, 4 unique 29mer shRNA constructs in retroviral untagged vector(Gene ID = 246721). 5µg purified plasmid DNA per construct 29-mer scrambled shRNA cassette in pRS Vector, TR30012, included for free.
RefSeq	NM_032958, NM_032959, NM_145325, NM_032959.1, NM_032959.2, NM_032959.3, NM_032959.4, NM_032959.5, NM_032958.3, NM_145325.2, BC086857, BC086857.1, BC050598, BC056864, BC071870, BC110985, NM_032959.6
Summary	This gene is a member of the RNA polymerase II subunit 11 gene family, which includes three genes in a cluster on chromosome 7q22.1 and a pseudogene on chromosome 7p13. The founding member of this family, DNA directed RNA polymerase II polypeptide J, has been shown to encode a subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. This locus produces multiple, alternatively spliced transcripts that potentially express isoforms with distinct C-termini compared to DNA directed RNA polymerase II polypeptide J. Most or all variants are spliced to include additional non-coding exons at the 3' end which makes them candidates for nonsense-mediated decay (NMD). Consequently, it is not known if this locus expresses a protein or proteins in vivo. [provided by RefSeq, Jul 2008]
shRNA Design	These shRNA constructs were designed against multiple splice variants at this gene locus. To be certain that your variant of interest is targeted, please contact techsupport@origene.com. If you need a special design or shRNA sequence, please utilize our custom shRNA service.
Performance Guaranteed	OriGene guarantees that the sequences in the shRNA expression cassettes are verified to correspond to the target gene with 100% identity. One of the four constructs at minimum are guaranteed to produce 70% or more gene expression knock-down provided a minimum transfection efficiency of 80% is achieved. Western Blot data is recommended over qPCR to evaluate the silencing effect of the shRNA constructs 72 hrs post transfection. To properly assess knockdown, the gene expression level from the included scramble control vector must be used in comparison with the target-specific shRNA transfected samples. For non-conforming shRNA, requests for replacement product must be made within ninety (90) days from the date of delivery of the shRNA kit. To arrange for a free replacement with newly designed constructs, please contact Technical Services at techsupport@origene.com. Please provide your data indicating the transfection efficiency and measurement of gene expression knockdown compared to the scrambled shRNA control (Western Blot data preferred).

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.