STAT6 Human shRNA Plasmid Kit (Locus ID 6778)

Specifications

Product Data
Product Name	STAT6 Human shRNA Plasmid Kit (Locus ID 6778)
Locus ID	6778
UniProt ID	P42226
Synonyms	D12S1644; IL-4-STAT; STAT6B; STAT6C
Vector	pRS
Format	Retroviral plasmids
Kit Components	STAT6 - Human, 4 unique 29mer shRNA constructs in retroviral untagged vector(Gene ID = 6778). 5µg purified plasmid DNA per construct 29-mer scrambled shRNA cassette in pRS Vector, TR30012, included for free.
RefSeq	NM_001178078, NM_001178079, NM_001178080, NM_001178081, NM_003153, NR_033659, NM_003153.1, NM_003153.2, NM_003153.3, NM_003153.4, NM_001178080.1, NM_001178081.1, NM_001178078.1, NM_001178079.1, BC075852, BC075852.1, BC004973, BC005823, NM_001178079.2, NM_003153.5, NM_001178078.2
Summary	The protein encoded by this gene is a member of the STAT family of transcription factors. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein plays a central role in exerting IL4 mediated biological responses. It is found to induce the expression of BCL2L1/BCL-X(L), which is responsible for the anti-apoptotic activity of IL4. Knockout studies in mice suggested the roles of this gene in differentiation of T helper 2 (Th2) cells, expression of cell surface markers, and class switch of immunoglobulins. Alternative splicing results in multiple transcript variants.[provided by RefSeq, May 2010]
shRNA Design	These shRNA constructs were designed against multiple splice variants at this gene locus. To be certain that your variant of interest is targeted, please contact techsupport@origene.com. If you need a special design or shRNA sequence, please utilize our custom shRNA service.
Performance Guaranteed	OriGene guarantees that the sequences in the shRNA expression cassettes are verified to correspond to the target gene with 100% identity. One of the four constructs at minimum are guaranteed to produce 70% or more gene expression knock-down provided a minimum transfection efficiency of 80% is achieved. Western Blot data is recommended over qPCR to evaluate the silencing effect of the shRNA constructs 72 hrs post transfection. To properly assess knockdown, the gene expression level from the included scramble control vector must be used in comparison with the target-specific shRNA transfected samples. For non-conforming shRNA, requests for replacement product must be made within ninety (90) days from the date of delivery of the shRNA kit. To arrange for a free replacement with newly designed constructs, please contact Technical Services at techsupport@origene.com. Please provide your data indicating the transfection efficiency and measurement of gene expression knockdown compared to the scrambled shRNA control (Western Blot data preferred).

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.