PAF Receptor (PTAFR) Human shRNA Plasmid Kit (Locus ID 5724)
CAT#: TR310087
PTAFR - Human, 4 unique 29mer shRNA constructs in retroviral untagged vector, 5µg of each construct provided
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CNY 4,790.00
货期*
现货
规格
Cited in 2 publications. |
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经常一起买 (2)
Specifications
Product Data | |
Product Name | PAF Receptor (PTAFR) Human shRNA Plasmid Kit (Locus ID 5724) |
Locus ID | 5724 |
UniProt ID | P25105 |
Synonyms | PAFR |
Vector | pRS |
Format | Retroviral plasmids |
Kit Components | PTAFR - Human, 4 unique 29mer shRNA constructs in retroviral untagged vector(Gene ID = 5724). 5µg purified plasmid DNA per construct29-mer scrambled shRNA cassette in pRS Vector, TR30012, included for free. |
RefSeq | NM_000952, NM_001164721, NM_001164722, NM_001164723, NM_000952.1, NM_000952.2, NM_000952.3, NM_000952.4, NM_001164721.1, NM_001164722.1, NM_001164722.2, NM_001164723.1, NM_001164723.2, BC063000, BC063000.1, BC013816, NM_001164722.3, NM_000952.5, NM_001164723.3 |
Summary | This gene encodes a seven-transmembrane G-protein-coupled receptor for platelet-activating factor (PAF) that localizes to lipid rafts and/or caveolae in the cell membrane. PAF (1-0-alkyl-2-acetyl-sn-glycero-3-phosphorylcholine) is a phospholipid that plays a significant role in oncogenic transformation, tumor growth, angiogenesis, metastasis, and pro-inflammatory processes. Binding of PAF to the PAF-receptor (PAFR) stimulates numerous signal transduction pathways including phospholipase C, D, A2, mitogen-activated protein kinases (MAPKs), and the phosphatidylinositol-calcium second messenger system. Following PAFR activation, cells become rapidly desensitized and this refractory state is dependent on PAFR phosphorylation, internalization, and down-regulation. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2011] |
shRNA Design | These shRNA constructs were designed against multiple splice variants at this gene locus. To be certain that your variant of interest is targeted, please contact techsupport@origene.com. If you need a special design or shRNA sequence, please utilize our custom shRNA service. |
Performance Guaranteed | OriGene guarantees that the sequences in the shRNA expression cassettes are verified to correspond to the target gene with 100% identity. One of the four constructs at minimum are guaranteed to produce 70% or more gene expression knock-down provided a minimum transfection efficiency of 80% is achieved. Western Blot data is recommended over qPCR to evaluate the silencing effect of the shRNA constructs 72 hrs post transfection. To properly assess knockdown, the gene expression level from the included scramble control vector must be used in comparison with the target-specific shRNA transfected samples. For non-conforming shRNA, requests for replacement product must be made within ninety (90) days from the date of delivery of the shRNA kit. To arrange for a free replacement with newly designed constructs, please contact Technical Services at techsupport@origene.com. Please provide your data indicating the transfection efficiency and measurement of gene expression knockdown compared to the scrambled shRNA control (Western Blot data preferred). |
Citations (2)
The use of this RNAi has been cited in the following citations: |
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Feed forward reciprocal activation of PAFR and STAT3 regulates epithelial-mesenchymal transition in non-small cell lung cancer
,Zhan, QM;Chen, J;Lan, T;Zhang, W;Dong, L;Kang, N;Zhang, S;Fu, M;Liu, B;Liu, K;,
Cancer Res.
,PubMed ID 26359459
[PTAFR]
|
Platelet-activating factor receptor-mediated PI3K/AKT activation contributes to the malignant development of esophageal squamous cell carcinoma
,Chen, J;Lan, T;Zhang, W;Dong, L;Kang, N;Zhang, S;Fu, M;Liu, B;Liu, K;Zhang, C;Hou, J;Zhan, Q;,
Oncogene
,PubMed ID 25639872
[PTAFR]
|
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