Macrophage Inflammatory Protein 1 beta (CCL4L2) Human shRNA Plasmid Kit (Locus ID 9560)

Specifications

Product Data
Product Name	Macrophage Inflammatory Protein 1 beta (CCL4L2) Human shRNA Plasmid Kit (Locus ID 9560)
Locus ID	9560
UniProt ID	Q8NHW4
Synonyms	AT744.2; CCL4L; LAG-1; LAG1; SCYA4L
Vector	pRS
Format	Retroviral plasmids
Kit Components	CCL4L2 - Human, 4 unique 29mer shRNA constructs in retroviral untagged vector(Gene ID = 9560). 5µg purified plasmid DNA per construct 29-mer scrambled shRNA cassette in pRS Vector, TR30012, included for free.
RefSeq	NM_001001435, NM_001291468, NM_001291469, NM_001291470, NM_001291471, NM_001291472, NM_001291473, NM_001291474, NM_001291475, NR_111970, NM_001001435.1, NM_001001435.2, NM_001291469.1, NM_001291470.1, NM_001291474.1, NM_001291473.1, NM_001291468.1, NM_001291472.1, NM_001291471.1, NM_001291475.1, BC092445, NM_001291468.2, NM_001291472.2, NM_001291475.2, NM_001291471.2, NM_001291469.2, NM_001291470.2, NM_001291473.2, NM_001291474.2
Summary	This gene is one of several cytokine genes that are clustered on the q-arm of chromosome 17. Cytokines are a family of secreted proteins that function in inflammatory and immunoregulatory processes. The protein encoded by this family member is similar to the chemokine (C-C motif) ligand 4 product, which inhibits HIV entry by binding to the cellular receptor CCR5. The copy number of this gene varies among individuals, where most individuals have one to five copies. This gene copy contains a non-consensus splice acceptor site at the 3' terminal exon found in other highly similar gene copies, and it thus uses other alternative splice sites for the 3' terminal exon, resulting in multiple transcript variants. [provided by RefSeq, Apr 2014]
shRNA Design	These shRNA constructs were designed against multiple splice variants at this gene locus. To be certain that your variant of interest is targeted, please contact techsupport@origene.com. If you need a special design or shRNA sequence, please utilize our custom shRNA service.
Performance Guaranteed	OriGene guarantees that the sequences in the shRNA expression cassettes are verified to correspond to the target gene with 100% identity. One of the four constructs at minimum are guaranteed to produce 70% or more gene expression knock-down provided a minimum transfection efficiency of 80% is achieved. Western Blot data is recommended over qPCR to evaluate the silencing effect of the shRNA constructs 72 hrs post transfection. To properly assess knockdown, the gene expression level from the included scramble control vector must be used in comparison with the target-specific shRNA transfected samples. For non-conforming shRNA, requests for replacement product must be made within ninety (90) days from the date of delivery of the shRNA kit. To arrange for a free replacement with newly designed constructs, please contact Technical Services at techsupport@origene.com. Please provide your data indicating the transfection efficiency and measurement of gene expression knockdown compared to the scrambled shRNA control (Western Blot data preferred).

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.