IL17RC Human shRNA Plasmid Kit (Locus ID 84818)

Specifications

Product Data
Product Name	IL17RC Human shRNA Plasmid Kit (Locus ID 84818)
Locus ID	84818
UniProt ID	Q8NAC3
Synonyms	CANDF9; IL17-RL; IL17RL
Vector	pRS
Format	Retroviral plasmids
Kit Components	IL17RC - Human, 4 unique 29mer shRNA constructs in retroviral untagged vector(Gene ID = 84818). 5µg purified plasmid DNA per construct 29-mer scrambled shRNA cassette in pRS Vector, TR30012, included for free.
RefSeq	NM_001203263, NM_001203264, NM_001203265, NM_032732, NM_153460, NM_153461, NM_153462, NM_153463, NR_037807, NM_153460.1, NM_153460.2, NM_153460.3, NM_153461.1, NM_153461.2, NM_153461.3, NM_032732.1, NM_032732.2, NM_032732.4, NM_032732.5, NM_001203265.1, NM_001203264.1, NM_001203263.1, NM_153463.1, BC006411, NM_001367278, NM_001367279, NM_001367280, NM_153461.4
Summary	This gene encodes a single-pass type I membrane protein that shares similarity with the interleukin-17 receptor (IL-17RA). Unlike IL-17RA, which is predominantly expressed in hemopoietic cells, and binds with high affinity to only IL-17A, this protein is expressed in nonhemopoietic tissues, and binds both IL-17A and IL-17F with similar affinities. The proinflammatory cytokines, IL-17A and IL-17F, have been implicated in the progression of inflammatory and autoimmune diseases. Multiple alternatively spliced transcript variants encoding different isoforms have been detected for this gene, and it has been proposed that soluble, secreted proteins lacking transmembrane and intracellular domains may function as extracellular antagonists to cytokine signaling. [provided by RefSeq, Feb 2011]
shRNA Design	These shRNA constructs were designed against multiple splice variants at this gene locus. To be certain that your variant of interest is targeted, please contact techsupport@origene.com. If you need a special design or shRNA sequence, please utilize our custom shRNA service.
Performance Guaranteed	OriGene guarantees that the sequences in the shRNA expression cassettes are verified to correspond to the target gene with 100% identity. One of the four constructs at minimum are guaranteed to produce 70% or more gene expression knock-down provided a minimum transfection efficiency of 80% is achieved. Western Blot data is recommended over qPCR to evaluate the silencing effect of the shRNA constructs 72 hrs post transfection. To properly assess knockdown, the gene expression level from the included scramble control vector must be used in comparison with the target-specific shRNA transfected samples. For non-conforming shRNA, requests for replacement product must be made within ninety (90) days from the date of delivery of the shRNA kit. To arrange for a free replacement with newly designed constructs, please contact Technical Services at techsupport@origene.com. Please provide your data indicating the transfection efficiency and measurement of gene expression knockdown compared to the scrambled shRNA control (Western Blot data preferred).

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.