Sirt1 Mouse shRNA Plasmid (Locus ID 93759)
CAT#: TR505485
Sirt1 - Mouse, 4 unique 29mer shRNA constructs in retroviral untagged vector, 5µg of each construct provided
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CNY 4,790.00
货期*
现货
规格
Cited in 2 publications. |
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经常一起买 (1)
Specifications
Product Data | |
Product Name | Sirt1 Mouse shRNA Plasmid (Locus ID 93759) |
Locus ID | 93759 |
UniProt ID | Q923E4 |
Synonyms | AA673258; S; Si; Sir2; Sir2a; Sir2alpha; SIR2L1 |
Vector | pRS |
Format | Retroviral plasmids |
Kit Components | Sirt1 - Mouse, 4 unique 29mer shRNA constructs in retroviral untagged vector(Gene ID = 93759). 5µg purified plasmid DNA per construct29-mer scrambled shRNA cassette in pRS Vector, TR30012, included for free. |
RefSeq | NM_001159589, NM_001159590, NM_019812, NM_019812.1, NM_019812.2, NM_019812.3, NM_001159589.1, NM_001159589.2, NM_001159590.1, BC152314, BC006584, BC165995 |
Summary | This gene encodes a member of the sirtuin family of proteins, characterized by their deacetylase activity and proposed role in longevity. The encoded protein regulates gene expression in a wide range of cell and tissue types through its NAD+-dependent deacetylation of histones, transcription factors and transcriptional coactivators. Brain-specific overexpression of this gene has been shown to result in increased median lifespan. Viability of homozygous knockout mice for this gene varies with strain background. Homozygous knockout mice of strains that do not exhibit embryonic lethality are sterile and have a reduced lifespan. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2015] |
shRNA Design | These shRNA constructs were designed against multiple splice variants at this gene locus. To be certain that your variant of interest is targeted, please contact techsupport@origene.com. If you need a special design or shRNA sequence, please utilize our custom shRNA service. |
Performance Guaranteed | OriGene guarantees that the sequences in the shRNA expression cassettes are verified to correspond to the target gene with 100% identity. One of the four constructs at minimum are guaranteed to produce 70% or more gene expression knock-down provided a minimum transfection efficiency of 80% is achieved. Western Blot data is recommended over qPCR to evaluate the silencing effect of the shRNA constructs 72 hrs post transfection. To properly assess knockdown, the gene expression level from the included scramble control vector must be used in comparison with the target-specific shRNA transfected samples. For non-conforming shRNA, requests for replacement product must be made within ninety (90) days from the date of delivery of the shRNA kit. To arrange for a free replacement with newly designed constructs, please contact Technical Services at techsupport@origene.com. Please provide your data indicating the transfection efficiency and measurement of gene expression knockdown compared to the scrambled shRNA control (Western Blot data preferred). |
Citations (2)
The use of this RNAi has been cited in the following citations: |
---|
Cathepsin Cleavage of Sirtuin 1 in Endothelial Progenitor Cells Mediates Stress-Induced Premature Senescence
,null,
The American Journal of Pathology
,PubMed ID 22234173
[Sirt1]
|
Dual regulation of TERT activity through transcription and splicing by ΔNP63α
,null,
Aging (Albany NY)
,PubMed ID 20157588
[Sirt1]
|
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