Ispd Mouse shRNA Plasmid (Locus ID 75847)

CAT#: TR513238

Ispd - Mouse, 4 unique 29mer shRNA constructs in retroviral untagged vector, 5µg of each construct provided



Need custom shRNA service?
Get a free quote

CNY 6,790.00


货期*
2周

规格
    • 1 kit

Product images

经常一起买 (2)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


ISPD Antibody - middle region
    • 100 ul

CNY 5,250.00

Specifications

Product Data
Product Name Ispd Mouse shRNA Plasmid (Locus ID 75847)
Locus ID 75847
UniProt ID Q5RJG7
Synonyms 4930579E17Rik; AV040780
Vector pRS
Format Retroviral plasmids
Kit Components Ispd - Mouse, 4 unique 29mer shRNA constructs in retroviral untagged vector(Gene ID = 75847). 5µg purified plasmid DNA per construct
29-mer scrambled shRNA cassette in pRS Vector, TR30012, included for free.
RefSeq BC086665, BC096030, BC118038, NM_001289502, NM_001289503, NM_001289504, NM_178629, NM_178629.1, NM_178629.2, NM_178629.3, NM_178629.4, NM_178629.5, NM_178629.6, NM_001289504.1, NM_001289503.1, NM_001289502.1, BC024061, BC049756, BC050993, BC118030, NM_001364305
Summary Cytidylyltransferase required for protein O-linked mannosylation (By similarity). Catalyzes the formation of CDP-ribitol nucleotide sugar from D-ribitol 5-phosphate (By similarity). CDP-ribitol is a substrate of FKTN during the biosynthesis of the phosphorylated O-mannosyl trisaccharide (N-acetylgalactosamine-beta-3-N-acetylglucosamine-beta-4-(phosphate-6-)mannose), a carbohydrate structure present in alpha-dystroglycan (DAG1), which is required for binding laminin G-like domain-containing extracellular proteins with high affinity (By similarity). Shows activity toward other pentose phosphate sugars and mediates formation of CDP-ribulose or CDP-ribose using CTP and ribulose-5-phosphate or ribose-5-phosphate, respectively (By similarity). Not Involved in dolichol production (By similarity).[UniProtKB/Swiss-Prot Function]
shRNA Design These shRNA constructs were designed against multiple splice variants at this gene locus. To be certain that your variant of interest is targeted, please contact techsupport@origene.com. If you need a special design or shRNA sequence, please utilize our custom shRNA service.
Performance Guaranteed OriGene guarantees that the sequences in the shRNA expression cassettes are verified to correspond to the target gene with 100% identity. One of the four constructs at minimum are guaranteed to produce 70% or more gene expression knock-down provided a minimum transfection efficiency of 80% is achieved. Western Blot data is recommended over qPCR to evaluate the silencing effect of the shRNA constructs 72 hrs post transfection. To properly assess knockdown, the gene expression level from the included scramble control vector must be used in comparison with the target-specific shRNA transfected samples.

For non-conforming shRNA, requests for replacement product must be made within ninety (90) days from the date of delivery of the shRNA kit. To arrange for a free replacement with newly designed constructs, please contact Technical Services at techsupport@origene.com. Please provide your data indicating the transfection efficiency and measurement of gene expression knockdown compared to the scrambled shRNA control (Western Blot data preferred).
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.
Customer Reviews 
Loading...