SRRM1 Human siRNA Oligo Duplex (Locus ID 10250)

Specifications

Product Data
Purity	HPLC purified
Quality Control	Tested by ESI-MS
Sequences	Available with shipment
Stability	One year from date of shipment when stored at -20°C.
# of transfections	Approximately 330 transfections/2nmol in 24-well plate under optimized conditions (final conc. 10 nM).
Note	Single siRNA duplex (10nmol) can be ordered.
Reference Data
RefSeq	NM_001303448, NM_001303449, NM_005839, NM_001366577, NM_001366589, NM_001366600, NM_001366566, NM_001366567, NM_001366569, NM_001366570, NM_001366572, NM_001366575, NM_001366576, NM_001366581, NM_001366582, NM_001366585, NM_001366587, NM_001366590, NM_001366592, NM_001366594, NM_001366595, NM_001366596, NM_001366597, NM_001366599, NR_159379, NR_159382, NR_159383, NR_159384, NR_159385, NR_159386, NR_159387, NR_159388, NR_159389, NR_159391, NM_001366565, NM_001366568, NM_001366571, NM_001366573, NM_001366578, NM_001366584, NM_001366586, NM_001366588, NM_001366591, NM_001366593, NM_001366598, NR_159378, NR_159380, NR_159390
Synonyms	160-KD; POP101; SRM160
Components	SRRM1 (Human) - 3 unique 27mer siRNA duplexes - 2 nmol each (Locus ID 10250) Included - SR30004, Trilencer-27 Universal Scrambled Negative Control siRNA Duplex - 2 nmol Included - SR30005, RNAse free siRNA Duplex Resuspension Buffer - 2 ml
Summary	Part of pre- and post-splicing multiprotein mRNP complexes. Involved in numerous pre-mRNA processing events. Promotes constitutive and exonic splicing enhancer (ESE)-dependent splicing activation by bridging together sequence-specific (SR family proteins, SFRS4, SFRS5 and TRA2B/SFRS10) and basal snRNP (SNRP70 and SNRPA1) factors of the spliceosome. Stimulates mRNA 3'-end cleavage independently of the formation of an exon junction complex. Binds both pre-mRNA and spliced mRNA 20-25 nt upstream of exon-exon junctions. Binds RNA and DNA with low sequence specificity and has similar preference for either double- or single-stranded nucleic acid substrates.[UniProtKB/Swiss-Prot Function]
Performance Guranteed	OriGene guarantees that at least two of the three Dicer-Substrate duplexes in the kit will provide at least 70% or more knockdown of the target mRNA when used at 10 nM concentration by quantitative RT-PCR when the TYE-563 fluorescent transfection control duplex (cat# SR30002) indicates that >90% of the cells have been transfected and the HPRT positive control (cat# SR30003) provides 90% knockdown efficiency. For non-conforming siRNA, requests for replacement product must be made within ninety (90) days from the date of delivery of the siRNA kit. To arrange for a free replacement with newly designed duplexes, please contact Technical Services at techsupport@origene.com. Please provide your data indicating the transfection efficiency and measurement of gene expression knockdown compared to the scrambled siRNA control (quantitative RT-PCR data required).

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.