Wnt4 Mouse siRNA Oligo Duplex (Locus ID 22417)

Specifications

Product Data
Purity	HPLC purified
Quality Control	Tested by ESI-MS
Sequences	Available with shipment
Stability	One year from date of shipment when stored at -20°C.
# of transfections	Approximately 330 transfections/2nmol in 24-well plate under optimized conditions (final conc. 10 nM).
Note	Single siRNA duplex (10nmol) can be ordered.
Reference Data
RefSeq	NM_009523
Synonyms	Wnt-4
Components	Wnt4 (Mouse) - 3 unique 27mer siRNA duplexes - 2 nmol each (Locus ID 22417) Included - SR30004, Trilencer-27 Universal Scrambled Negative Control siRNA Duplex - 2 nmol Included - SR30005, RNAse free siRNA Duplex Resuspension Buffer - 2 ml
Summary	Ligand for members of the frizzled family of seven transmembrane receptors (Probable). Plays an important role in the embryonic development of the urogenital tract and the lung (PubMed:7990960, PubMed:9989404, PubMed:16054034, PubMed:17537789, PubMed:19830824, PubMed:26321050). Required for normal mesenchyme to epithelium transition during embryonic kidney development (PubMed:7990960, PubMed:16054034, PubMed:17537789, PubMed:19830824). Required for the formation of early epithelial renal vesicles during kidney development (PubMed:16054034). Required for normal formation of the Mullerian duct in females, and normal levels of oocytes in the ovaries (PubMed:9989404, PubMed:19830824). Required for normal down-regulation of 3 beta-hydroxysteroid dehydrogenase in the ovary (PubMed:9989404). Required for normal lung development and for normal patterning of trachael cartilage rings (PubMed:26321050).[UniProtKB/Swiss-Prot Function]
Performance Guranteed	OriGene guarantees that at least two of the three Dicer-Substrate duplexes in the kit will provide at least 70% or more knockdown of the target mRNA when used at 10 nM concentration by quantitative RT-PCR when the TYE-563 fluorescent transfection control duplex (cat# SR30002) indicates that >90% of the cells have been transfected and the HPRT positive control (cat# SR30003) provides 90% knockdown efficiency. For non-conforming siRNA, requests for replacement product must be made within ninety (90) days from the date of delivery of the siRNA kit. To arrange for a free replacement with newly designed duplexes, please contact Technical Services at techsupport@origene.com. Please provide your data indicating the transfection efficiency and measurement of gene expression knockdown compared to the scrambled siRNA control (quantitative RT-PCR data required).

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.