Rif1 Mouse siRNA Oligo Duplex (Locus ID 51869)

CAT#: SR423488

Rif1 (Mouse) - 3 unique 27mer siRNA duplexes - 2 nmol each



Need single siRNA in bulk?
Get a free quote

CNY 4,090.00


货期*
7周

规格
    • 1 kit

Product images

经常一起买 (3)
siTran 2.0 siRNA transfection reagent (0.5ml)
    • 500 ul

CNY 2,190.00


Trilencer-27 Fluorescent-labeled transfection control siRNA duplex - 1 nmol
    • 1 nmol

CNY 1,090.00


Rabbit polyclonal anti-Rif1 antibody
    • 100 ug

CNY 8,025.00

Specifications

Product Data
Purity HPLC purified
Quality Control Tested by ESI-MS
Sequences Available with shipment
Stability One year from date of shipment when stored at -20°C.
# of transfections Approximately 330 transfections/2nmol in 24-well plate under optimized conditions (final conc. 10 nM).
Note Single siRNA duplex (10nmol) can be ordered.
Reference Data
RefSeq NM_175238, NM_001355414, NM_001355415
Synonyms 5730435J01Rik; 6530403D07Rik; AU016181; AW549474; D2Ertd145e
Components Rif1 (Mouse) - 3 unique 27mer siRNA duplexes - 2 nmol each (Locus ID 51869)
Included - SR30004, Trilencer-27 Universal Scrambled Negative Control siRNA Duplex - 2 nmol
Included - SR30005, RNAse free siRNA Duplex Resuspension Buffer - 2 ml
Summary Key regulator of TP53BP1 that plays a key role in the repair of double-strand DNA breaks (DSBs) in response to DNA damage: acts by promoting non-homologous end joining (NHEJ)-mediated repair of DSBs (PubMed:23333305, PubMed:23306437, PubMed:23306439). In response to DNA damage, interacts with ATM-phosphorylated TP53BP1 (PubMed:23333305, PubMed:23306437, PubMed:23306439). Interaction with TP53BP1 leads to dissociate the interaction between NUDT16L1/TIRR and TP53BP1, thereby unmasking the tandem Tudor-like domain of TP53BP1 and allowing recruitment to DNA DSBs (By similarity). Once recruited to DSBs, RIF1 and TP53BP1 act by promoting NHEJ-mediated repair of DSBs (PubMed:23333305, PubMed:23306437). In the same time, RIF1 and TP53BP1 specifically counteract the function of BRCA1 by blocking DSBs resection via homologous recombination (HR) during G1 phase (PubMed:23333305, PubMed:23306437). Also required for immunoglobulin class-switch recombination (CSR) during antibody genesis, a process that involves the generation of DNA DSBs (PubMed:23333305, PubMed:23333306, PubMed:23306439). Promotes NHEJ of dysfunctional telomeres (PubMed:23333305).[UniProtKB/Swiss-Prot Function]
Performance Guranteed OriGene guarantees that at least two of the three Dicer-Substrate duplexes in the kit will provide at least 70% or more knockdown of the target mRNA when used at 10 nM concentration by quantitative RT-PCR when the TYE-563 fluorescent transfection control duplex (cat# SR30002) indicates that >90% of the cells have been transfected and the HPRT positive control (cat# SR30003) provides 90% knockdown efficiency.

For non-conforming siRNA, requests for replacement product must be made within ninety (90) days from the date of delivery of the siRNA kit. To arrange for a free replacement with newly designed duplexes, please contact Technical Services at techsupport@origene.com. Please provide your data indicating the transfection efficiency and measurement of gene expression knockdown compared to the scrambled siRNA control (quantitative RT-PCR data required).
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Documents

Customer Reviews 
Loading...