pAAVS1-Cas9-Puro-DNR

CAT＃: GE100037

Cas9 expression vector in AAVS1 donor vector, puromycin selection (10 ug)

Datasheet
SDS
Vector Sequence

CN¥ 7,510.00

货期*
4周
规格
    • 10 ug
Cited in 2 publications.

Product images

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TP790148
Purified recombinant protein of S. pyogenes Cas9 endonuclease (Cas9) containing Simian virus 40 (SV40) T antigen nuclear localization sequence (NLS) on the N- and C- termini of the protein., with N-terminal HIS tag, expressed in E.coli, sterile-filtered, suitable for cell culture
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TP790151
Purified recombinant protein of mutant(D10A) of S. pyogenes Cas9 endonuclease (Cas9) containing Simian virus 40 (SV40) T antigen nuclear localization sequence (NLS) on the N- and C- termini of the protein., with N-terminal HIS tag, expressed in E. coli
    • 500 pmol

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CC100003
DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CN¥ 1,280.00

暂停销售

Specifications

Product Data
Function Cas9 only vectors
Features

Cas9 in AAVS1-Puro donor vector

  • CMV-Cas9 and PGK-Puro in AAVS1 donor vector.
  • Contains AAVS1 left and right homologous sequences (550 bp each side)
  • CMV-Cas9 and PGK-Puro cassette can be integrated into human AAVS1 site when cotransfected with pCas-Guide-AAVS1
  • When transfected into cells alone, can be used as regular Cas9 mammalian expression vector
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Citations (2)

The use of this Vectors has been cited in the following citations:
Safe harbor-targeted CRISPR-Cas9 homology-independent targeted integration for multimodality reporter gene-based cell tracking ,Kelly, JJ;Saee-Marand, M;Nyström, NN;Evans, MM;Chen, Y;Martinez, FM;Hamilton, AM;Ronald, JA;, Science advances ,PubMed ID 33523917 [pAAVS1-Cas9-Puro-DNR]
Safe Harbor Targeted CRISPR-Cas9 Tools for Molecular-Genetic Imaging of Cells in Living Subjects ,Dubois, V;Zotova, D;Parkins, K;Swick, C;Hamilton, A;Kelly, J;Ronald, J;, The CRISPR Journal [pAAVS1-Cas9-Puro-DNR]

Documents

Product Manuals
FAQs
SDS
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DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)

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