pCMV6-AC-DDK Mammalian Expression Vector

CAT＃: PS100005

pCMV6-AC-DDK, mammalian vector with C-terminal DDK tag, 10ug

Datasheet
SDS
Vector Sequence

CNY 5,420.00

货期*
现货
规格
    • 10 ug
Cited in 12 publications.

Product images

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Specifications

Product Data
C-Tag DDK
E. coli Selection Ampicillin (100 ug/mL)
Cell Selection Neomycin
Features
  • ORFs cloned in this vector will be expressed as a tagged protein with a C-terminal DDK-tag. (DDK is the same as FLAG® which is a registered trademark of Sigma Aldrich).
  • Such clones are the best for detection and purification of the transgene expression using an anti-DDK antibody.
  • If the C-terminal tagging interferes with the protein’s function, you can choose the N-terminal DDK-tagging vector, PS100014.
  • V2_F Primer can be used to sequence the target sequence after cloning.
  • XL39_R Primer can be used to sequence the target sequence after cloning.
Download Vector Sequence
Schematic of the multiple cloning sites
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Citations (12)

The use of this Vectors has been cited in the following citations:
Ultrarare Loss-of-Function Mutations in the Genes Encoding the Ionotropic Glutamate Receptors of Kainate Subtypes Associated with Schizophrenia Disrupt the Interaction with PSD95 ,null, Journal of Personalized Medicine ,PubMed ID 35629206
RTEL1 influences the abundance and localization of TERRA RNA ,null, Nature Communications ,PubMed ID 34021146
TRAP1 chaperone protein mutations and autoinflammation ,Standing, AS;Hong, Y;Paisan-Ruiz, C;Omoyinmi, E;Medlar, A;Stanescu, H;Kleta, R;Rowcenzio, D;Hawkins, P;Lachmann, H;McDermott, MF;Eleftheriou, D;Klein, N;Brogan, PA;, Life Sci Alliance ,PubMed ID 31882397 [pCMV6-AC-DDK]
GLK-IKKβ signaling induces dimerization and translocation of the AhR-RORγt complex in IL-17A induction and autoimmune disease ,Chuang, HC;Tsai, CY;Hsueh, CH;Tan, TH;, Sci Adv ,PubMed ID 30214937 [pCMV6-AC-DDK]
Dominant-negative IKZF1 mutations cause a T, B, and myeloid cell combined immunodeficiency ,Boutboul, D;Kuehn, HS;Van de Wyngaert, Z;Niemela, JE;Callebaut, I;Stoddard, J;Lenoir, C;Barlogis, V;Farnarier, C;Vely, F;Yoshida, N;Kojima, S;Kanegane, H;Hoshino, A;Hauck, F;Lhermitte, L;Asnafi, V;Roehrs, P;Chen, S;Verbsky, JW;Calvo, KR;Husami, A;Zhang, K;Roberts, J;Amrol, D;Sleaseman, J;Hsu, AP;Holland, SM;Marsh, R;Fischer, A;Fleisher, TA;Picard, C;Latour, S;Rosenzweig, SD;, J. Clin. Invest. ,PubMed ID 29889099 [pCMV6-AC-DDK]
Mitochondrial Ca2+ Uniporter is a Mitochondrial Luminal Redox Sensor That Augments MCU Channel Activity ,null, Molecular cell ,PubMed ID 28262504
Hypoxia-induced epithelial-mesenchymal transition is regulated by phosphorylation of GSK3-β via PI3 K/Akt signaling in oral squamous cell carcinoma ,null, Oral Surgery, Oral Medicine, Oral Pathology and Oral Radiology ,PubMed ID 27614812
T cell proliferation and adaptive immune responses are critically regulated by protein phosphatase 4 ,Liao, FH;Hsiao, WY;Lin, YC;Chan, YC;Huang, CY;, Cell Cycle ,PubMed ID 26940341
TRAF2-mediated Lys63-linked ubiquitination of DUSP14/MKP6 is essential for its phosphatase activity ,Yang, CY;Chiu, LL;Tan, TH;, Cell. Signal. ,PubMed ID 26521044 [PS100005]
The phosphatase JKAP/DUSP22 inhibits T-cell receptor signalling and autoimmunity by inactivating Lck ,Li, JP;Yang, CY;Chuang, HC;Lan, JL;Chen, DY;Chen, YM;Wang, X;Chen, AJ;Belmont, JW;Tan, TH;, Nat Commun 3618 5 . April 2014 ,PubMed ID 24714587 [pCMV6-AC-DDK]
Characterization of the genomic features and expressed fusion genes in micropapillary carcinomas of the breast ,Natrajan, R;Wilkerson, PM;Marchiò, C;Piscuoglio, S;Ng, CK;Wai, P;Lambros, MB;Samartzis, EP;Dedes, KJ;Frankum, J;Bajrami, I;Kopec, A;Mackay, A;A'hern, R;Fenwick, K;Kozarewa, I;Hakas, J;Mitsopoulos, C;Hardisson, D;Lord, CJ;Kumar-Sinha, C;Ashworth, A;Weigelt, B;Sapino, A;Chinnaiyan, AM;Maher, CA;Reis-Filho, JS;, J. Pathol., Jan 2014. ,PubMed ID 24395524 [pCMV6-AC-DDK]
Dual-Specificity Phosphatase 14 (DUSP14/MKP6) Negatively Regulates TCR Signaling by Inhibiting TAB1 Activation ,Yang, CY;Li, JP;Chiu, LL;Lan, JL;Chen, DY;Chuang, HC;Huang, CY;Tan, TH;, J. Immunol., Jan 2014. ,PubMed ID 24403530 [pCMV6-AC-Flag]

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