pCMV6-A-Puro Mammalian Expression Vector

CAT＃: PS100025

pCMV6-A-Puro, mammalian vector with puromycin as mammalian selection marker, 10ug

Datasheet
SDS
Vector Sequence

CNY 5,420.00

货期*
现货
规格
    • 10 ug
Cited in 21 publications.

Product images

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Forward primer to sequence targets cloned in expression vectors.
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Specifications

Product Data
E. coli Selection Ampicillin (100 ug/mL)
Cell Selection Puromycin
Features
  • This vector is essentially the same as the pCMV6-Entry vector, except for the alternative selection marker (Puromycin instead of Neomycin).
  • The vector itself does not contain additional tag. If the insert is shuttled from pCMV6-Entry via SgfI/AscI and RsrII/MluI, it will express non-tagged protein. If the insert is shuttled from the pCMV6-Entry vector using SgfI/AscI and Pme I, then it will express Myc-DDK tagged protein.
  • V2_F Primer can be used to sequence the target sequence after cloning.
  • XL39_R Primer can be used to sequence the target sequence after cloning.
Download Vector Sequence
Schematic of the multiple cloning sites
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Citations (21)

The use of this Vectors has been cited in the following citations:
PHAROH lncRNA regulates Myc translation in hepatocellular carcinoma via sequestering TIAR ,null, eLife ,PubMed ID 34002693
Migraine therapeutics differentially modulate the CGRP pathway ,Bhakta, M;Vuong, T;Taura, T;Wilson, DS;Stratton, JR;Mackenzie, KD;, Cephalalgia ,PubMed ID 33626922 [pCMV6-A-Puro]
VU0606170, a Selective Slack Channels Inhibitor, Decreases Calcium Oscillations in Cultured Cortical Neurons ,Spitznagel, B;Mishra, N;Qunies, A;Prael, F;Du, Y;Kozek, K;Lazarenko, R;Denton, J;Emmitte, K;Weaver, C;, ACS Chem. Neurosci. ,PubMed ID 33143429 [pCMV6-A-Puro]
Coupled transmembrane mechanisms control MCU-mediated mitochondrial Ca2+ uptake ,null, Proceedings of the National Academy of Sciences of the United States of America ,PubMed ID 32801213
A Novel Van der Woude Syndrome-Causing IRF6 Variant Is Subject to Incomplete Non-sense-Mediated mRNA Decay Affecting the Phenotype of Keratinocytes ,null, Frontiers in Cell and Developmental Biology ,PubMed ID 33117810
Ovarian granulosa cell tumor characterization identifies FOXL2 as an immunotherapeutic target ,Pierini, S;Tanyi, JL;Simpkins, F;George, E;Uribe-Herranz, M;Drapkin, R;Burger, R;Morgan, MA;Facciabene, A;, JCI Insight ,PubMed ID 32814714 [pCMV6-A-Puro]
NADPH oxidase 1 is highly expressed in human large and small bowel cancers ,null, PLoS ONE ,PubMed ID 32428030
Carbohydrate (Chondroitin 4) Sulfotransferase-11-Mediated Induction of Epithelial-Mesenchymal Transition and Generation of Cancer Stem Cells. ,null, Pharmacology ,PubMed ID 32344408
Codon optimization is an essential parameter for the efficient allotopic expression of mtDNA genes ,Lewis, CJ;Dixit, B;Batiuk, E;Hall, CJ;O'Connor, MS;Boominathan, A;, Redox Biol ,PubMed ID 31981894 [pCMV6-A-Puro]
Exon 2 Skipping Eliminates Gamma-Glutamyl Carboxylase Activity, Indicating a Partial Splicing Defect in a Patient with Vitamin K Clotting Factor Deficiency ,Rishavy, MA;Hallgren, KW;Zhang, H;Runge, KW;Berkner, KL;, J. Thromb. Haemost. ,PubMed ID 31009158 [pCMV6-A-Puro]
Discovery and Characterization of VU0529331, a Synthetic Small-Molecule Activator of Homomeric G Protein-gated, Inwardly-rectifying, Potassium (GIRK) Channels ,null, ACS chemical neuroscience ,PubMed ID 30136838
The teneurin C-terminal domain possesses nuclease activity and is apoptogenic ,Ferralli, J;Tucker, RP;Chiquet-Ehrismann, R;, Biol Open ,PubMed ID 29555638 [pCMV6-A-Puro]
The role of progesterone and conceptus-derived factors in uterine biology during early pregnancy in ruminants ,Spencer, TE;Forde, N;Lonergan, P;, J. Dairy Sci ,PubMed ID 26387021 [pCMV6-A-Puro]
Dual inhibition of ErbB1 and ErbB2 in cancer by recombinant human prolidase mutant hPEPD-G278D ,Yang, L;Li, Y;Bhattacharya, A;Zhang, Y;, Oncotarget ,PubMed ID 27286447 [pCMV6-A-Puro]
KLF4a stimulates breast cancer cell proliferation by acting as a KLF4 antagonist ,Ferralli, J;Chiquet-Ehrismann, R;Degen, M;, Oncotarget ,PubMed ID 27323810
Endotoxin tolerance in monocytes can be mitigated by a2-interferon ,Shi, L;Song, L;Maurer, K;Sharp, J;Zhang, Z;Sullivan, KE;, J. Leukoc. Biol. ,PubMed ID 26206900 [PS100025]
Development and Validation of a Thallium Flux-Based Functional Assay for the Sodium Channel NaV1.7 and Its Utility for Lead Discovery and Compound Profiling ,Du, Y;Days, E;Romaine, I;Abney, KK;Kaufmann, K;Sulikowski, G;Stauffer, S;Lindsley, CW;Weaver, CD;, ACS Chem Neurosci ,PubMed ID 25879403 [PS100025]
Interferon regulatory factor 1 and histone H4 acetylation in systemic lupus erythematosus ,null, Epigenetics ,PubMed ID 25611806
IRF1 marks activated genes in SLE and can induce target gene expression ,Zhang, Z;Shi, L;Song, L;Ephrem, E;Petri, M;E Sullivan, K;, Arthritis & rheumatology ,PubMed ID 25418955 [Others]
p53-Independent Cell Cycle and Erythroid Differentiation Defects in Murine Embryonic Stem Cells Haploinsufficient for Diamond Blackfan Anemia-Proteins: RPS19 versus RPL5 ,Singh, SA;Goldberg, TA;Henson, AL;Husain-Krautter, S;Nihrane, A;Blanc, L;Ellis, SR;Lipton, JM;Liu, JM;, PLoS ONE, Feb 2014. ,PubMed ID 24558476 [pCMV6-A-Puro]
Mule/Huwe1/Arf-BP1 suppresses Ras-driven tumorigenesis by preventing c-Myc/Miz1-mediated down-regulation of p21 and p15 ,Satoshi Inoue, Zhenyue Hao, Andrew J. Elia, David Cescon, Lily Zhou, Jennifer Silvester, Bryan Snow, Isaac S. Harris, Masato Sasaki, Wanda Y. Li, Momoe Itsumi, Kazuo Yamamoto, Takeshi Ueda, Carmen Dominguez-Brauer, Chiara Gorrini, Iok In Christine Chio, Jillian Haight, Annick You-Ten, Susan McCracken, Andrew Wakeham, Danny Ghazarian, Linda J.Z. Penn, Gerry Melino, and Tak W. Mak, Genes & Dev., May 2013; 27: 1101 - 1114. ,PubMed ID 23699408 [pCMV6-A-Puro]

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TurboFectin Transfection Reagent (1 mL in 1 vial)

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    • 5 x 200 ul

CNY 1,280.00 暂停销售

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