TrueORF^® cDNA Clones: Ready-to-Use Tagged cDNA Clones

TrueORF cDNA clones are tagged ORF clones in mammalian expression vectors

• Genome wide coverage for human, mouse and rat
• Two types of tags: Myc-DDK tagged or GFP tagged
• New: Lenti options are now available.
• 20,000 Human clones are validated for protein expression
• 70 destination vectors for additional applications
• Clone collections available

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The same ORF inserts are available in 4 different expression vectors

	pCMV6-Entry	pCMV6-AC-GFP	pLenti-C-Myc-DDK	pLenti-C-mGFP
Vector SKU	PS100001	PS100010	PS100064	PS100071
Vector Map
Fusion Tag	C-terminal Myc-DDK	C-terminal tGFP	C-terminal Myc-DDK	C-terminal mGFP
Ideal For	Detection and pufirication of over-expressed protein	Tracking the over-expressed protein in tranfected cells	In hard-to-transfect cells: Detection and purification of over-expressed protein	Tracking the over-expressed protein in tranfected cells

Technical features of TrueORF:

• The CMV promoter and a Kozak consensus sequence drive protein expression in mammalian cells.
• The C-terminus of the ORF is tagged with Myc/DDK* or tGFP.
• Ten (10) ug of purified plasmid is provided for immediate transfection and expression experiments.
• The upstream T7 promoter allows protein expression in cell-free system.
• Engineered as the entry vector for the OriGene PrecisionShuttle system.

Each TrueORF Clone is provided with a pair of sequencing primers:

View Location on Vectors

* Peptide sequence of the DDK-tag (Flag®): N-DYKDDDDK-C Flag® is a registered trademark of Sigma-Aldrich