E Cadherin (CDH1) (incl. pos. control) Mouse Monoclonal Antibody [Clone ID: 22F8]
CAT#: AM00010BT-N
E Cadherin (CDH1) (incl. pos. control) mouse monoclonal antibody, clone 22F8, Biotin
Conjugation: Unconjugated
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CNY 6,798.00
货期*
5周
规格
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Specifications
Product Data | |
Clone Name | 22F8 |
Applications | WB |
Recommend Dilution | Immunoblotting: 0.5 µg/ml for HRPO/ECL detection. Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubation buffer. Included Potitive Control: Cell lysate from untreated A431 (See Protocols for more details). |
Reactivity | Human |
Host | Mouse |
Clonality | Monoclonal |
Immunogen | Peptide conjugated to hemocyanin. Epitope: Cytoplasmic domain. |
Specificity | This monoclonal antibody specifically recognizes E-Cadherin at 124 kDa. |
Formulation | PBS containing 0.09% Sodium Azide, PEG and Sucrose. Label: Biotin State: Liquid purified IgG fraction. |
Concentration | lot specific |
Purification | Subsequent Ultrafiltration and Size Exclusion Chromatography. |
Conjugation | Biotin |
Storage Condition | Aliquote and freeze in liquid nitrogen. Antibody can be stored frozen at -80°C up to 1 year. Thaw aliquots at 37°C. Thawed aliquots may be stored at 4°C up to 3 months. |
Gene Name | cadherin 1 |
Database Link | |
Background | Cadherins are a family of transmembrane glycoproteins that play a key role in calcium-dependent cell-cell adhesion. Several members of the cadherin family have been identified so far, including E-(epithelial), P-(placental), N-(neuronal), and M- muscle) cadherin. E-cadherin is expressed in most epithelial tissues. |
Synonyms | Epithelial cadherin, E-cadherin, Uvomorulin, CAM 120/80, CDH1, CDHE, UVO |
Note | Protocol: Positive Control: Cell lysate from untreated A431 cells, epidermoid carcinoma (Human) Format: Lyophilized cell lysate from serum starved A431 cells. Reconstitution: Restore by addition of 200 µl H2O. After complete solubilization add 200 µl 2x SDS-PAGE sample buffer, mix and incubate at 90°C for 5 min. Application: The positive control cell lysate is recommended for immunoblot applications. 20 µl of positive control cell lysate correspond to ca. 20.000 cells. Use 20 µl/lane (mini gel) for HRPO/ECL detection of the target proteins. Please NOTE: The lyophilized cell lysates conatin SDS and are not recommended for applications with native proteins such as in immunoprecipitation. Storage: Aliquote reconstituted product and store frozen. Avoid repeated fereezing and thawing. |
Reference Data | |
Protein Families | Druggable Genome, ES Cell Differentiation/IPS, Transmembrane |
Protein Pathways | Adherens junction, Bladder cancer, Cell adhesion molecules (CAMs), Endometrial cancer, Melanoma, Pathogenic Escherichia coli infection, Pathways in cancer, Thyroid cancer |
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