E Cadherin (CDH1) (incl. pos. control) Mouse Monoclonal Antibody [Clone ID: 22F8]

CAT#: AM00010BT-N

E Cadherin (CDH1) (incl. pos. control) mouse monoclonal antibody, clone 22F8, Biotin

Conjugation: Unconjugated Biotin



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CNY 6,798.00


货期*
5周

规格
    • 100 ug

Product images

Specifications

Product Data
Clone Name 22F8
Applications WB
Recommend Dilution Immunoblotting: 0.5 µg/ml for HRPO/ECL detection.
Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubation buffer.
Included Potitive Control: Cell lysate from untreated A431 (See Protocols for more details).
Reactivity Human
Host Mouse
Clonality Monoclonal
Immunogen Peptide conjugated to hemocyanin.
Epitope: Cytoplasmic domain.
Specificity This monoclonal antibody specifically recognizes E-Cadherin at 124 kDa.
Formulation PBS containing 0.09% Sodium Azide, PEG and Sucrose.
Label: Biotin
State: Liquid purified IgG fraction.
Concentration lot specific
Purification Subsequent Ultrafiltration and Size Exclusion Chromatography.
Conjugation Biotin
Storage Condition Aliquote and freeze in liquid nitrogen.
Antibody can be stored frozen at -80°C up to 1 year.
Thaw aliquots at 37°C.
Thawed aliquots may be stored at 4°C up to 3 months.
Gene Name cadherin 1
Background Cadherins are a family of transmembrane glycoproteins that play a key role in calcium-dependent cell-cell adhesion. Several members of the cadherin family have been identified so far, including E-(epithelial), P-(placental), N-(neuronal), and M- muscle) cadherin. E-cadherin is expressed
in most epithelial tissues.
Synonyms Epithelial cadherin, E-cadherin, Uvomorulin, CAM 120/80, CDH1, CDHE, UVO
Note Protocol: Positive Control: Cell lysate from untreated A431 cells, epidermoid carcinoma (Human)

Format: Lyophilized cell lysate from serum starved A431 cells.

Reconstitution: Restore by addition of 200 µl H2O. After complete solubilization add 200 µl 2x SDS-PAGE sample buffer, mix and incubate at 90°C for 5 min.

Application: The positive control cell lysate is recommended for immunoblot applications. 20 µl of positive control cell lysate correspond to ca. 20.000 cells.
Use 20 µl/lane (mini gel) for HRPO/ECL detection of the target proteins.
Please NOTE: The lyophilized cell lysates conatin SDS and are not recommended for applications with native proteins such as in immunoprecipitation.

Storage: Aliquote reconstituted product and store frozen. Avoid repeated fereezing and thawing.
Reference Data
Protein Families Druggable Genome, ES Cell Differentiation/IPS, Transmembrane
Protein Pathways Adherens junction, Bladder cancer, Cell adhesion molecules (CAMs), Endometrial cancer, Melanoma, Pathogenic Escherichia coli infection, Pathways in cancer, Thyroid cancer
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.
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