E Cadherin (CDH1) (incl. pos. control) Mouse Monoclonal Antibody [Clone ID: 22F8]

Specifications

Product Data
Clone Name	22F8
Applications	WB
Recommend Dilution	Immunoblotting: 0.5 μg/ml for HRPO/ECL detection. Recommended buffer: Casein/Tween 20 based blocking and blot incubation buffer. Use cell lysate from untreated A431 cells as positive control.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Peptide conjugated to hemocyanin. Epitope: cytoplasmic domain.
Specificity	This antibody specifically recognizes E-cadherin at 124 kDa.
Formulation	2 x PBS / 0.09% sodium azide / PEG and sucrose State: Purified State: Lyophilized Ig fraction
Reconstitution Method	Restore with 1 ml H2O (15 min, RT).
Purification	The antibody was purified from serum-free cell culture supernatant by subsequent ultrafiltration and size exclusion chromatography.
Conjugation	Unconjugated
Storage Condition	For long-term storage, freeze lyophilizate upon arrival (-20°C). Upon reconstitution, aliquote and freeze in liquid nitrogen; reconstituted antibody can be stored frozen at -80°C up to 1 year. Thaw aliquots at 37°C. Thawed aliquots may be stored at 4°C up to 3 months. Avoid repeated freezing and thawing.
Gene Name	cadherin 1
Database Link	Entrez Gene 999 Human UniProt ID P12830
Background	Cadherins are a family of transmembrane glycoproteins that play a key role in calcium-dependent cell-cell adhesion. Several members of the cadherin family have been identified so far, including E-(epithelial), P-(placental), N-(neuronal), and M- muscle) cadherin. E-cadherin is expressed in most epithelial tissues.
Synonyms	Epithelial cadherin, E-cadherin, Uvomorulin, CAM 120/80, CDH1, CDHE, UVO
Note	Cell lysate from untreated A431 cells as positive control is supplied with this product. Protocol: Positive control provided: Control cell lysate A 431 untreated Format: Lyophilized cell lysate from serum starved A431 cells. Reconstitute by addition of 200 µl H2O. After complete solubilization add 200 µl 2x SDS-PAGE sample buffer, mix and incubate at 90°C for 5 min. Applications: The positive control cell lysate is recommended for immunoblot applications. 20 ml of positive control cell lysate correspond to ca. 20.000 cells. Use 20 µl / lane (mini gel) for HRPO/ECL detection of the target proteins. Please note: The lyophilized cell lysates contain SDS and are not recommended for applications with native proteins such as immunoprecipitation. Storage: In aliquots at -20°C. Avoid repeated freezing and thawing.
Reference Data

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