beta Catenin (CTNNB1) pTyr654 (incl. pos. control) Mouse Monoclonal Antibody [Clone ID: 1B11]

Specifications

Product Data
Clone Name	1B11
Applications	WB
Recommend Dilution	Western Blot: approx. 1 μg/ml for HRPO/ECL detection. Recommended buffer: Casein/Tween 20 based blocking and blot incubation buffer. Positive control: Cell lysate from pervanadate-treated SW480 cells (please see: 'Protocols')
Reactivity	Canine, Human, Mouse
Host	Mouse
Clonality	Monoclonal
Immunogen	Phosphopeptide conjugated to hemocyanin Epitope: phosphotyrosine 654 / 643 (V A T pY A A A).
Specificity	This antibody specifically recognizes β-catenin phosphorylated at tyrosine 654 at 90 kDa and gamma-catenin at 70 kDa. Due to sequence homologies of the phospho-epitope surrounding pY 654 there may be crossreactions with other proteins (e.g. Annexin A11 at 56 kDa, gamma-catenin at 70 kDa, Desmoplakin at 81 kDa, TNFalpha-induced protein 2 at 73 kDa, Ribosomal protein S6 kinase alpha 6 at 90 kDa).
Formulation	2 x PBS containing 0.09 % Na-azide, PEG and Sucrose. State: Purified State: Lyophilized Ig fraction
Reconstitution Method	Restore with 1 ml H2O (15 min, RT).
Purification	Subsequent thiophilic adsorption and size exclusion chromatography
Conjugation	Unconjugated
Storage Condition	For long-term storage, freeze lyophilizate upon arrival (-20°C). Upon reconstitution, aliquote and freeze in liquid nitrogen; reconstituted antibody should be stored frozen at -80°C. Avoid repeated freeze-thaw cycles.
Gene Name	catenin beta 1
Database Link	Entrez Gene 1499 Human UniProt ID P35222
Background	The α-, β- and γ-catenins are cytoplasmic proteins mediating the interaction of Ca2+-dependent transmembrane adhesion molecules (cadherins) with the cytoskeletal network. Additionally, β-catenin is a substrate of both receptor and non-receptor tyrosine kinases. Tyrosine 86 and tyrosine 654 are substrates of EGF receptor and src family kinases while tyrosine 142 is a substrate of fer tyrosine kinases.
Synonyms	CTNNB1, CTNNB, Beta-catenin
Note	Positive control cell lysate is included. Protocol: Positive Control Cell Lysate: SW 480 PERVANADATE TREATED Format: Lyophilized cell lysate from SW480 cells Serum starved cells were treated for 15 min with pervanadate. Reconstitution: Restore by addition of 200 µl H2O. After complete solubilization add 200 µl 2x SDS-PAGE sample buffer, mix and incubate at 90°C for 5 min. Application: The positive control cell lysate is recommended for immunoblot applications. 20 µl of positive control cell lysate correspond to ca. 20.000 cells. Use 20 µl/lane (mini gel) for HRPO/ECL detection of the target proteins. Please NOTE: The lyophilized cell lysates conatin SDS and are not recommended for applications with native proteins such as in immunoprecipitation. Storage: Aliquote reconstituted product and store frozen. Avoid repeated fereezing and thawing.
Reference Data

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