EGFR pTyr869 (incl. pos. control) Mouse Monoclonal Antibody [Clone ID: 12A3]

Specifications

Product Data
Clone Name	12A3
Applications	ELISA, IF, IHC, IP, WB
Recommend Dilution	Western Blot: 1 µg/ml for HRPO/ECL detection. Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubation buffer. ELISA: 0.1 µg/ml (protein ELISA). Immunoprecipitation: 1 - 10 μg per 106 vanadate treated A431 cells. Immunocytochemistry: 1 - 10 μg/ml. Immunohistochemistry on frozen and paraffin sections. Luminex.
Reactivity	Human, Mouse
Host	Mouse
Clonality	Monoclonal
Immunogen	Phosphopeptide conjugated to KLH.
Specificity	This antibody specifically recognizes EGFR phosphorylated at Tyrosine 869 and detects EGFR activation after interaction with src kinases. Mab 12A3 does not crossreact with the highly homologues pTyr 877 of activated erbB2.
Formulation	PBS/0.09% Na-Azide/PEG and Sucrose Label: Biotin State: Liquid
Concentration	lot specific
Purification	Size exclusion chromatography
Conjugation	Biotin
Storage Condition	Store the antibody (aliquote in liquid nitrogen) at -80°C. Avoid repeated freezing and thawing. Thaw aliquots at 37°C. Thawed aliquots may be stored at 4°C up to 3 months.
Gene Name	epidermal growth factor receptor
Database Link	Entrez Gene 1956 Human UniProt ID P00533
Background	EGFR/erbB receptors are activated upon binding of EGF and EGF-related growth factors such as TGF alpha, beta-cellulin, Hb-EGF, HRG, or NRG. Binding of these ligands leads to receptor homo- and heterodimerization followed by autophosphorylation and activation of downstream signal transduction pathways (MAPK, PI3K/PKB, and STAT). In addition, EGFR becomes fully activated after phosphorylation of Y869 by src family kinases. Phosphorylation of Y1069 leads to association with cbl and subsequent receptor degradation. Phosphorylation of S1071 by CamKinase II leads to attenuation of kinase activity; phosphorylation of T678 (by PKC) and T693 (by MAPK, p38) interferes with receptor endocytosis/recycling.
Synonyms	Epidermal growth factor receptor, EGF Receptor, erbB-1, c-ErbB-1
Reference Data

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