Vimentin (VIM) (incl. pos. control) Mouse Monoclonal Antibody [Clone ID: 11H6]

Specifications

Product Data
Clone Name	11H6
Applications	WB
Recommend Dilution	Western Blot: 0.5 µg/ml for HRPO/ECL detection. Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubation buffer.  Included Positive Control: Cell lysate from Untreated HeLa cells (See Protocols for more details).
Reactivity	Canine, Human, Mouse
Host	Mouse
Clonality	Monoclonal
Immunogen	Synthetic peptide conjugated to hemocyanin.  Epitope: N-terminus.
Specificity	This antibody specifically recognizes Vimentin at 54 kDa in Western-blot applications.
Formulation	1ml 2 x PBS containing 0.09% Sodium Azide/PEG and Sucrose. State: Purified State: Lyophilized purified IgG fraction.
Reconstitution Method	Restore with 1 ml H2O (15 min, RT).
Purification	Subsequent Ultrafiltration and Size Exclusion Chromatography.
Conjugation	Unconjugated
Storage Condition	Store lyophilized (preferably in a desiccator) at -20°C and reconstituted (aliquote and freeze in liquid nitrogen) at -80°C. Avoid repeated freezing and thawing. Thaw aliquots at 37°C. Thawed aliquots may be stored at 4°C up to 3 months.
Predicted Protein Size	54 kDa
Gene Name	vimentin
Database Link	Entrez Gene 7431 Human UniProt ID P08670
Background	Vimentin is a major subunit protein of intermediate filaments found in mesenchymal cells. Vimentin is expressed in many tumors/tumor cell lines after epithelial to mesenchymal transition.
Synonyms	VIM
Note	Protocol: Positive Control Provided: Cell lysate from Untreated HeLa cells.   Lyophilized cell lysate from serum starved HeLa cells. Format: Lyophilized cell lysate from HeLa cells. Reconstitution: Restore by addition of 200 μl H20. After complete solubilization add 200 μl 2x SDS-PAGE sample buffer, mix and incubate at 90°C for 5 min.  Storage: Aliquote and store frozen. Avoid repeated freeze/thaw cycles.  Application: The positive control cell lysate is recommended for immunoblot applications. 20 μl of positive control cell lysate correspond to ca. 80.000 cells. Use 20 μl / lane (mini gel) for HRPO/ECL detection of the target proteins.  Please note: The lyophilized cell lysates contain SDS and are not recommended for applications with native proteins such as immunoprecipitation.
Reference Data

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