Phosphotyrosine Mouse Monoclonal Antibody [Clone ID: PY20]

Specifications

Product Data
Clone Name	PY20
Applications	ELISA
Recommend Dilution	ELISA: 1/1000 - 1/4000.
Reactivity	Broad
Host	Mouse
Clonality	Monoclonal
Immunogen	U266 cell line.
Specificity	This antibody recognises phosphotyrosine, enabling the detection, characterisation and isolation of proteins containing phosphorylated tyrosine residues. The phosphorylation of tyrosine acts as a important signal in the control of cell mitogenesis, differentiation, proliferation, and migration and occurs following the activation of intracellular tyrosine kinases, including the Tcell receptor (TCR), epidermal growth factor (EGF) and many families of receptor and non-receptor protein tyrosine kinases (PTKs), which catalyse the transfer of ATP to a tyrosine residue on specific cell protein targets. The binding of PY20 to phosphorylated tyrosines can be inhibited by free phosphotyrosine and phenylphosphate, but not by free phosphate, phosphoserine or phosphothreonine.
Formulation	50%PBS, pH7.4 and 50% Glycerol Label: HRP State: Liquid purified IgG Label: Horseradish Peroxidase
Purification	Affinity chromatography
Conjugation	HRP
Storage Condition	Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Reference Data

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