Phosphotyrosine Mouse Monoclonal Antibody [Clone ID: PY20]

Specifications

Product Data
Clone Name	PY20
Applications	ELISA, IF, IHC, IP, WB
Recommend Dilution	Suitable for Western Blot, ELISA, Immunohistochemistry and Immunocytochemistry. Working dilution of 1/2,000 is suggested for Western blot or ELISA, 1/500 for Immunohistochemistry or Immunocytochemistry.
Reactivity	Broad
Host	Mouse
Clonality	Monoclonal
Immunogen	Hybridoma produced from Balb/C mice immunized with phosphotyrosine coupled to carrier protein.
Specificity	Clone PY20 specifically recognizes phosphorylated tyrosine residues and does not react with phosphorylated threonine or serine residues.
Formulation	50% glycerol, 0.02 M sodium phosphate, pH 7.5, 0.15 M sodium chloride. Label: HRP State: Liquid purified IgG fraction Label: Coupled through free primary amino groups to Periodate-activated Horseradish Peroxidase The binding site of the antibody was protected by the addition of phenyl phosphate prior to coupling
Concentration	lot specific
Purification	Affinity purification on a column of immobilized phosphotyrosine
Conjugation	HRP
Storage Condition	Store the antibody (undiluted) at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Background	The role of tyrosine phosphorylation in transduction of the mitogenic signal from transmembrane receptors and in transformation by oncogene tyrosine kinases has been the subject of intense investigation for several years, While the phosphorylation of specific tyrosine residues has be shown to be a primary mechanism of signal transduction during normal mitogenesis, cell cycle progression and oncogenic transformation, its role in other areas such as differentiation and gap junction communication, is a matter of active and ongoing research. Antibodies that specifically recognize phosphorylated tyrosine residues have proved invaluable to the study of tyrosine-phosphorylated proteins and the biochemical pathways in which they fuction. The HRP conjugate of PY20 anti-phosphotyrosine is especailly useful for the detection of phosphotyrosyl proteins in western blotting, immunohistochemical and immuncytochemical protcols in situations wherin the use of a secondary antibody would produce undesirable background signals.
Reference Data

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