IL2RA Mouse Monoclonal Antibody [Clone ID: IL-A111]

Specifications

Product Data
Clone Name	IL-A111
Applications	FC
Recommend Dilution	Flow cytometry (Neat - 1/5): use 10 µl of the suggested working dilution to label 1x10e6 cells in 100 µl.
Reactivity	Bovine, Sheep
Host	Mouse
Clonality	Monoclonal
Specificity	This antibody recognises the bovine CD25 cell surface antigen, a 55 kDa glycoprotein also known as Interleukin-2 receptor alpha chain, which is expressed by activated T cells. Cross reacts with sheep. Other species not tested.
Formulation	PBS, pH 7.4 containing 0.09% Sodium Azide and 1% Bovine Serum Albumin Label: FITC State: Liquid purified IgG Label: Fluorescein Isothiocyanate Isomer 1
Concentration	lot specific
Purification	Affinity chromatography on Protein G
Conjugation	FITC
Storage Condition	Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. This product is photosensitive and should be protected from light.
Database Link	Entrez Gene 281861 Bovine UniProt ID P12342
Background	The Interleukin 2 Receptor alpha and beta chains, together with the common gamma chain, constitute the high affinity IL2 receptor present on activated T and B cells, thymocyte subset, pre B cells and T regulatory cells. Homodimeric alpha chains result in low affinity receptor, while homodimeric beta chains produce a medium affinity receptor. Normally an integral membrane protein, soluble IL2 Receptor alpha has been isolated and determined to result from extracellular proteolysis. Alternately spliced IL2 Receptor alpha mRNAs have been isolated, but the significance of each is presently unknown.
Synonyms	Interleukin-2 receptor alpha chain, IL-2 receptor alpha subunit, IL-2-RA, IL2-RA, p55, TAC antigen
Note	Clone IL-A111 is reported to block the IL-2 driven proliferation of Con A-induced blast cells/ bovine lymphocytes. Removal of sodium azide is recommended prior to use in functional assays.
Reference Data

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