Complement C5 (C5) Mouse Monoclonal Antibody [Clone ID: 10-04]

Specifications

Product Data
Clone Name	10-04
Applications	ELISA, FN, WB
Recommend Dilution	ELISA. Western Blot. Functional Assays.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Purified Human C5
Specificity	This antibody recognizes secreted complement component 5 (C5), a 190kDa serine protease present in blood serum. In haemolysis inhibition studies it was found that this antibody will block C5 haemolytic activity. Removal of Sodium Azide is recommended prior to use in Functional Assays.
Formulation	Borate buffered saline State: Purified State: Liquid purified Ig fraction Preservative: 0.09% Sodium Azide
Concentration	lot specific
Purification	Affinity Chromatography on Protein A
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	complement component 5
Database Link	Entrez Gene 727 Human UniProt ID P01031
Background	It is composed of an 115kDa disulphide linked alpha-chain and a 75 kDa beta-chain. In addition to its role in complement, C5 is thought to be associated with inflammation, stimulating tumour necrosis factor alpha, interferon gamma mediated suppression of IL10 and IL12 production. C5 is activated via either the classical or the alternative complement pathway, by proteolytic cleavage of C5a from the N-terminal of the alpha-chain, by C5 convertase. C5a is an anaphylotoxin which mediates local inflammation. It induces the contraction of smooth muscle, increases vascular permeability and causes histamine release from mast cells and basophilic leukocytes. It also stimulates chemotaxis of neutrophils, eosinophils, basophils and monocytes towards the site of inflammation. The remaining fragment, C5b, initiates the assembly of the membrane attack complex.
Synonyms	CPAMD4, Complement component 5
Reference Data
Protein Families	Druggable Genome
Protein Pathways	Complement and coagulation cascades, Prion diseases, Systemic lupus erythematosus

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