Cytokeratin 18 (KRT18) Mouse Monoclonal Antibody [Clone ID: RGE53]

Specifications

Product Data
Clone Name	RGE53
Applications	IF, IHC, WB
Recommend Dilution	Immunoblotting. Immunofluorescence. Immunohistochemistry on Frozen Sections. Working Dilution: 1/5-1/10, dilute immediately before use with PBS or TBS. Recommended Positive Control: Adenocarcinoma.
Reactivity	Canine, Chicken, Human, Mammalian, Mouse, Porcine, Rabbit, Rat, Birds
Host	Mouse
Clonality	Monoclonal
Immunogen	Isolated from HeLa cells
Specificity	The antibody is useful for the discrimination of adenocarcinomas and mesotheliomas from squamous cell carcinomas and non-epithelial tumors. Clone RGE 53 specifically recognizes simple and glandular epithelial cells from the gastrointestinal tract, the respiratory tract and the urogenital tract, as well as endocrine and exocrine tissues and myoepithelial cells. In the transitional epithelium of the bladder RGE 53 reacts only with superficial (umbrella) cells. No reaction with stratified squamous epithelia. Polypeptide Reacting: 45 kD Keratin K18 (formerly also designated Cytokeratin 18).
Formulation	State: Supernatant State: Liquid Culture Supernatant containing 0.09% Sodium Azide
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	keratin 18
Database Link	Entrez Gene 3875 Human UniProt ID P05783
Background	Cytokeratin 18 is an acidic keratin which is found primarily in non squamous epithelia and is present in a majority of adenocarcinomas and ductal carcinomas but not in squamous cell carcinomas. Cytokeratin 18 exists in combination with Cytokeratin 8, a basic keratin. Hepatocellular carcinomas have been reportedly defined by the use of antibodies that recognize only Cytokeratins 8 and 18.
Synonyms	Cytokeratin-18, CK18, Keratin-18, Keratin 18, KRT18, CYK18, K18
Reference Data

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