SHIP (INPP5D) Mouse Monoclonal Antibody [Clone ID: SHIP-01]

Specifications

Product Data
Clone Name	SHIP-01
Applications	ELISA, WB
Recommend Dilution	Flow Cytometry (2-5 μg/ml) Positive control: human blood leukocytes as Western Blotting (Reducing conditions) Positive control: RAMOS human cell line. Sample preparation: Resuspend approx. 50 mil. cells in 1 ml cold Lysis buffer (1% laurylmaltoside in 20 mM Tris/Cl, 100 mM NaCl pH 8.2, 50 mM NaF including Protease inhibitor Cocktail). Incubate 60 min on ice. Centrifuge to remove cell debris. Mix lysate with reducing Laemmli SDS-PAGE sample buffer. Boil for 6 min. ELISA.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Peptide coresponding to a sequence within N-terminal domain of Human SHIP-1.
Specificity	The antibody SHIP-01 reacts with SHIP-1, a phosphoinositide phosphatase largely confined to hematopoietic cells. Multiple forms of SHIP-1 have been reported with molecular weights of 110, 125, 130, 135 and 145 kDa.
Formulation	PBS, pH 7.4 with 15 mM sodium azide as preservative. State: Aff - Purified State: Liquid purified Ig fraction (> 95% pure by SDS-PAGE).
Concentration	lot specific
Purification	Protein A affinity chromatography.
Conjugation	Unconjugated
Storage Condition	Store the antibody at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	inositol polyphosphate-5-phosphatase D
Database Link	Entrez Gene 3635 Human UniProt ID Q92835
Background	SHIP-1 (SH2 domain containing inositol phosphatase-1) is a 5´inositol phosphatase that regulates cell responses in lymphocytes and myeloid cells by hydrolyzing the second messenger PI(3,4,5) trisphosphate. SHIP-1 is recruited upon engagement of both inhibitory and activatory receptors, such as FcgammaRIIB, Fcgamma RIII, FcepsilonRI or cytokine and growth factor receptors, and supresses PI3K-dependent signaling, down-regulates cell migration and invasion of transformed cells and phagocytosis. SHIP-1 also serves as a scaffold for the recruitment of other proteins to the plasma membrane.
Synonyms	SHIP, SHIP1, SHIP-1, p150Ship, SIP-145
Reference Data
Protein Families	Druggable Genome
Protein Pathways	B cell receptor signaling pathway, Fc epsilon RI signaling pathway, Fc gamma R-mediated phagocytosis, Insulin signaling pathway, Phosphatidylinositol signaling system

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