CD300 antigen (CD300A) Mouse Monoclonal Antibody [Clone ID: MEM-260]

Specifications

Product Data
Clone Name	MEM-260
Applications	FC, WB
Recommend Dilution	Flow Cytometry: 2 µg/ml. Western Blot (Non-reducing conditions). Positive material: HPB cell line; Negative material: JURKAT human cell line, RAJI human cell line. Sample preparation: Resuspend approx. 50 mil. cells in 1 ml cold Lysis buffer (1% laurylmaltoside in 20 mM Tris/Cl, 100 mM NaCl pH 8.2, 50 mM NaF including Protease inhibitor Cocktail). Incubate 60 min on ice. Centrifuge to remove cell debris. Mix lysate with non-reducing SDS-PAGE sample buffer.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	HPB human acute lymphoid leukemia cell line
Specificity	This antibody reacts with CD300a, a 60 kDa leukocyte transmembrane glycoprotein expressed on human granulocytes, monocytes, neutrophils, NK cells, mast cells and dendritic cells, 25% of circulating T cells and 15% of circulating B cells.
Formulation	PBS, pH 7.4 containing 15 mM sodium azide as preservative State: Purified State: Liquid purified IgG fraction (> 95% pure by SDS-PAGE)
Concentration	lot specific
Purification	Protein A affinity chromatography
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C. DO NOT FREEZE!
Gene Name	CD300a molecule
Database Link	Entrez Gene 11314 Human UniProt ID Q9UGN4
Background	CD300a (CMRF-35H, IRp60) is a non-MHC-specific inhibitory receptor of immunoglobulin superfamily, which contains three immunoreceptor tyrosine-based inhibitory motifs (ITIMs) that associate with SH2-containing phosphatases SHP-1 and SHP-2. CD300a is expressed on many cell types including T cells, NK cells, neutrophils, eosinophils or mast cells. Its triggering inhibits activating signals such as those of IL5, GM-CSF or eotaxin, as well as supresses mast cell degranulation or NK cell cytotoxic activity.
Synonyms	CLM-8, CMRF35-H, CMRF35-H9, CMRF-35-H9, IRC1/IRC2, IRp60
Reference Data

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