p53 (TP53) pSer392 Mouse Monoclonal Antibody [Clone ID: FP3.2]

Specifications

Product Data
Clone Name	FP3.2
Applications	IHC, WB
Recommend Dilution	Western Blotting: 1 μg/ml. Immunohistochemistry on Paraffin Sections: 10 μg/ml. Staining technique: Standard ABC technique (DAB+) Pretreatment: High temperature antigen retrieval (microwave, pressure cooker) in 10 mM citrate buffer pH 6.0 or 1 mM EDTA-NaOH buffer pH 8.0. Incubation Time: 1 hour at RT or overnight at 4°C Positive Tissue: breast carcinoma with high level of wild-type p53.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	KLH-conjugated phosphopeptide RHKKLMFKTEGPDS[P] corresponding to amino acids 378-393 representing the C-terminus of Human p53.
Specificity	This p53 antibody FP3.2 [FPS392] reacts with Human p53 tumour suppressor protein phosphorylated at CKII site (Ser392).
Formulation	PBS, pH~7.4 State: Aff - Purified State: Liquid purified IgG fraction (> 95% pure by SDS-PAGE) Preservative: 15 mM Sodium Azide
Concentration	lot specific
Purification	Protein A Affinity Chromatography
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	tumor protein p53
Database Link	Entrez Gene 7157 Human UniProt ID P04637
Background	The tumour suppressor protein p53 is a key element of intracellular anticancer protection. It mediates cell cycle arrest or apoptosis in response to DNA damage or to starvation for pyrimidine nukleotides. It is up-regulated in response to these stress signals and stimulated to activate transcription of specific genes, resulting in expression of p21waf1 and other proteins involved in G1 or G2/M arrest, or proteins that trigger apoptosis, such as Bcl-2. The structure of p53 comprises N-terminal transactivation domain, central DNA-binding domain, oligomerisation domain, and C-terminal regulatory domain. There are various phosphorylation sites on p53, of which the phosphorylation at Ser15 is important for p53 activation and stabilization.
Synonyms	Cellular tumor antigen p53, Tumor suppressor p53, Phosphoprotein p53, NY-CO-13
Note	Predicted Molecular Weight: 53 kDa
Reference Data

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