Stromal interaction molecule 1 (STIM1) Mouse Monoclonal Antibody [Clone ID: CDN3H4]

Specifications

Product Data
Clone Name	CDN3H4
Applications	IF, IHC, IP, WB
Recommend Dilution	Immunoprecipitation. Western Blotting (reducing and non-reducing conditions): 1 μg/ml Positive Control: RBL rat basophilic leukemia cell line Sample Preparation: Resuspend approx. 50 mil. cells in 1 ml cold Lysis buffer (1% laurylmaltoside in 20 mM Tris/Cl, 100 mM NaCl pH 8.2, 50 mM NaF including Protease inhibitor Cocktail). Incubate 60 min on ice. Centrifuge to remove cell debris. Mix lysate with non-reducing/reducing Laemmli SDS-PAGE sample buffer. Immunocytochemistry: Staining Technique: methanol-aceton fixation. Positive Control: HeLa human cervix carcinoma cell line. Immunohistochemistry on Paraffin Sections: 5 μg/ml.  Heat induced antigen retrieval in pH 6.0 citrate buffer is recommended.
Reactivity	Human, Mouse, Rat
Host	Mouse
Clonality	Monoclonal
Immunogen	Synthetized peptide (C-terminal cytoplasmic part of STIM1)
Specificity	The antibody CDN3H4 reacts with Human and Rodent STIM1, a 84 kDa essential and conserved regulator of store-operated Ca2+ channel function.
Formulation	PBS, pH 7.4 State: Aff - Purified State: Liquid purified IgG fraction (> 95% pure by SDS-PAGE) Preservative: 15 mM Sodium Azide
Concentration	lot specific
Purification	Affinity Chromatography on Protein A
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C. DO NOT FREEZE!
Gene Name	stromal interaction molecule 1
Database Link	Entrez Gene 6786 Human UniProt ID Q13586
Background	STIM1 (stromal interacting molecule; also known as GOK) acts as a sensor of calcium depletion within the endoplasmic reticulum and transduces the signal to Orai1, the presumptive CRAC channel at the plasma membrane. Following decrease of luminal calcium concentration, STIM1 oligomerizes and induces Orai1 to enable entry of extracellular calcium into the cytoplasm. However, the precise mechanism of STIM1-Orai1 interaction has not been elucidated yet. Many questions also remain to be solved around STIM1 functional distribution. It turns out that STIM1 associates with growing ends of microtubules and is involved in endoplasmic reticulum tubule extension.
Synonyms	Stromal interaction molecule 1
Reference Data

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