CD73 (NT5E) (27-252) Mouse Monoclonal Antibody [Clone ID: AT89E9]

Specifications

Product Data
Clone Name	AT89E9
Applications	ELISA, FC, IF, IHC, WB
Recommend Dilution	ELISA. Flow Cytometry. Western blot: 1/1,000-1/2,000. Immunofluorescence/Immnunocytochemistry.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Recombinant Human CD73 (aa 27-252) purified from E. coli
Specificity	This antibody is specific for Human NT5E/CD73. Other species not tested.
Formulation	PBS, pH 7.4 containing 0.02% Sodium Azide and 10% Glycerol State: Purified State: Liquid purified Ig fraction
Concentration	lot specific
Purification	Protein-G affinity chromatography
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C for up to two weeks or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	5'-nucleotidase ecto
Database Link	Entrez Gene 4907 Human UniProt ID P21589
Background	CD73 (Ecto-5'-nucleotidase, 5'-NT) is a membrane-bound glycoprotein that functions to hydrolyze extracellular nucleoside monophosphates into bioactive nucleoside intermediates. This enables the uptake of adenosine, inosine, and guanosine into the cell and their subsequent reconversion into ATP and GTP in the purine salvage pathway. The expression of CD73 on neuronal cells has been described during developmental processes and, on lymphocytes, CD73 serves as a maturation marker, being absent from the surface of both immature B and T cells.
Synonyms	NT5, NTE, NT5E
Reference Data

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