Calpain (CAPN1+CAPN2) Mouse Monoclonal Antibody [Clone ID: 28F3]
CAT#: AM05265PU-N
Calpain (CAPN1+CAPN2) mouse monoclonal antibody, clone 28F3, Purified
Conjugation: Agarose
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CNY 5,937.00
货期*
4周
规格
Cited in 1 publication. |
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Specifications
Product Data | |
Clone Name | 28F3 |
Applications | ELISA, IP, WB |
Recommend Dilution | ELISA: 0.5-1 ug/ml. Western blot: 0.5-1 ug/ml. Immunoprecipitation of both the native and denatured protein: 1-2 ug/ml. |
Reactivity | Bovine, Human |
Host | Mouse |
Clonality | Monoclonal |
Immunogen | Calpain purified from human placenta. |
Specificity | This antibody specifically recognizes the 28-30 kD subunit of m-calpain and u-calpain. React with human and bovine calpains, but does not recognize calpains from rat or mouse. |
Formulation | 0.02 M Sodium Phosphate, pH 7.5, 0.15 M Sodium Chloride, 50% Glycerol and 3 mM Sodium Azide as presevtative. State: Purified State: Liquid (sterile filtered) purified IgG fraction (> 95% pure). |
Concentration | lot specific |
Purification | Standard chromatographic techniques. |
Conjugation | Unconjugated |
Storage Condition | Store the antibody at -20°C. Avoid repeated freezing and thawing. |
Background | The calpains are calcium-dependent cysteine proteases that are widely expressed in mammalian systems. Both m-calpain (calpain II) and -calpain (calpain I) are composed of an 80 kD subunit and a 30 kD subunit. Whereas the 30 kDa subunit is shared by both enzymes, the larger catalytic subunits are different and exhibit the distinct Ca ++ requirements that are suggested by their names. Whereas m-calpain requires millimolar (mM) levels of calcium, u-calpain is active at micromolar (uM) concentrations of Ca++. In addition to the ubiquitously expressed m- and u-calpains, some tissue-specific calpains have been identified. The calpains appear to serve multiple physiological roles, and ideas concerning the functions of these enzymes are in a state of rapid flux. |
Synonyms | CAPN2, CANPL2, CANP-2, CAPN1, CANPL1, CANP1 |
Reference Data |
Citations (1)
The use of this Antibodies has been cited in the following citations: |
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Distinct Roles for ?-Calpain and m-Calpain in Synaptic NMDAR-Mediated Neuroprotection and Extrasynaptic NMDAR-Mediated Neurodegeneration
,null,
The Journal of Neuroscience
,PubMed ID 24285894
|
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