Calpain (CAPN1+CAPN2) Mouse Monoclonal Antibody [Clone ID: 28F3]

CAT#: AM05265PU-N

Calpain (CAPN1+CAPN2) mouse monoclonal antibody, clone 28F3, Purified

Conjugation: Unconjugated Agarose



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CNY 5,937.00


货期*
4周

规格
    • 100 ug

Cited in 1 publication.

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Specifications

Product Data
Clone Name 28F3
Applications ELISA, IP, WB
Recommend Dilution ELISA: 0.5-1 ug/ml.
Western blot: 0.5-1 ug/ml.
Immunoprecipitation of both the native and denatured protein: 1-2 ug/ml.
Reactivity Bovine, Human
Host Mouse
Clonality Monoclonal
Immunogen Calpain purified from human placenta.
Specificity This antibody specifically recognizes the 28-30 kD subunit of m-calpain and u-calpain.
React with human and bovine calpains, but does not recognize calpains from rat or mouse.
Formulation 0.02 M Sodium Phosphate, pH 7.5, 0.15 M Sodium Chloride, 50% Glycerol and 3 mM Sodium Azide as presevtative.
State: Purified
State: Liquid (sterile filtered) purified IgG fraction (> 95% pure).
Concentration lot specific
Purification Standard chromatographic techniques.
Conjugation Unconjugated
Storage Condition Store the antibody at -20°C.
Avoid repeated freezing and thawing.
Background The calpains are calcium-dependent cysteine proteases that are widely expressed in mammalian systems. Both m-calpain (calpain II) and -calpain (calpain I) are composed of an 80 kD subunit and a 30 kD subunit. Whereas the 30 kDa subunit is shared by both enzymes, the larger catalytic subunits are different and exhibit the distinct Ca ++ requirements that are suggested by their names. Whereas m-calpain requires millimolar (mM) levels of calcium, u-calpain is active at micromolar (uM) concentrations of Ca++. In addition to the ubiquitously expressed m- and u-calpains, some tissue-specific calpains have been identified. The calpains appear to serve multiple physiological roles, and ideas concerning the functions of these enzymes are in a state of rapid flux.
Synonyms CAPN2, CANPL2, CANP-2, CAPN1, CANPL1, CANP1
Reference Data
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Citations (1)

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