Caspase 3 (CASP3) Mouse Monoclonal Antibody [Clone ID: AM1-4]
CAT#: AM05282PU-N
Caspase 3 (CASP3) mouse monoclonal antibody, clone AM1-4, Purified
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CNY 5,937.00
货期*
4周
规格
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Specifications
Product Data | |
Clone Name | AM1-4 |
Applications | WB |
Recommend Dilution | Western Blot. |
Reactivity | Human |
Host | Mouse |
Clonality | Monoclonal |
Immunogen | Hybridoma produced by the fusion of splenocytes from mice immunized with recombinant human Caspase-3 protein and mouse myeloma cells. |
Specificity | This antibody Detects human Caspase-3. |
Formulation | PBS containing 0.08% Sodium Azide as preservative. State: Purified State: Liquid (sterile filtered) purified IgG fraction. |
Concentration | lot specific |
Conjugation | Unconjugated |
Storage Condition | Store the antibody at -20°C. Avoid repeated freezing and thawing. |
Gene Name | caspase 3 |
Database Link | |
Background | Caspase-3 along with caspase 7 and 6 form the group of effector caspases that are responsible for the cleavage of multiple substrates including the cytokeratins, PARP, alpha fodrin, NuMA and others. Caspase-7 occurs in three varient forms.Caspase-3-like activities are required for Fas-mediated apoptosis. However, the role of caspase-1 and caspase-3 in mediating Fas-induced cell death is not clear. Although wild-type, caspase-1(-/-), and caspase-3(-/-) hepatocytes were killed at a similar rate when cocultured with FasL expressing NIH 3T3 cells, caspase-3(-/-) hepatocytes displayed drastically different morphological changes as well as significantly delayed DNA fragmentation. For both wild-type and caspase-1 (-/-) apoptotic hepatocytes, typical apoptotic features such as cytoplasmic blebbing and nuclear fragmentation are seen within 6 hr, but neither event was observed for caspase-3(-/-) hepatocytes. In thymocytes apoptotic caspase-3 (-/-) thymocytes exhibit similar abnormal morphological changes and delayed DNA fragmentation observed in hepatocytes. Cleavage of various caspase substrates implicates apoptotic events, including gelsolin, fodrin, laminB, and DFF45/ICAD are delayed or absent. The altered cleavage of these key substrates is likely responsible for the aberrant apoptosis observed in both hepatocytes and thymocytes deficient in caspase-3. |
Synonyms | CASP-3, CASP3, CPP32, CPP-32, Yama protein, Apopain, SCA-1, SCA1 |
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