MITF Mouse Monoclonal Antibody [Clone ID: C5]

Specifications

Product Data
Clone Name	C5
Applications	EMSA, IHC, IP, WB
Recommend Dilution	Gel Supershift. Western Blot (1 µg/ml). Immunoprecipitation (2 µg/mg of protein lysate). Immunohistochemistry on Frozen and Paraffin Sections. Positive Control: 501 Mel Human melanoma cells, wild-type Human, Rat, Mouse osteoclast cells.
Reactivity	Human, Mouse, Rat
Host	Mouse
Clonality	Monoclonal
Immunogen	Hybridoma produced by the fusion of splenocytes from RBF/DnJ mice immunized with an N-terminal fragment of Human microphthalmia protein and mouse myeloma NS1 cells.
Specificity	Recognizes in Western blotting a doublet of 52-56kDa, identified as serine-phosphorylated and unphosphorylated forms of melanocytic isoforms of microphthalmia (Mi) transcription factor. This antibody does not cross-react with other b-HLH-ZIP factors by DNA mobility shift assay.
Formulation	PBS containing 0.08% Sodium Azide as preservative State: Purified State: Liquid purified IgG fraction
Concentration	lot specific
Purification	Protein A/G Chromatography
Conjugation	Unconjugated
Storage Condition	Upon receipt, store undiluted (in aliquots) at -20°C. Avoid repeated freezing and thawing.
Predicted Protein Size	52-56 kDa
Gene Name	melanogenesis associated transcription factor
Database Link	Entrez Gene 4286 Human UniProt ID O75030
Background	There are two known isoforms of MiTF differing by 66 amino acids at the NH2 terminus. Shorter forms are expressed in melanocytes and run as two bands at 52kDa and 56kDa, while the longer Mi form runs as a cluster of bands at 60-70kDa in osteoclasts and in B16 melonoma cells (but not other melanoma cell lines), as well as mast cells and heart. It reacts with both melanocytic as well as the non- melanocytic isoforms of MiTF. Mi is a basic helix-loop-helix-leucin zipper (b-HLH-ZIP) transtripotion factor implicated in pigmentation, mast cells and bone development. The mutation of MiTF causes Waardenburg Syndrome type II in humans. In mice, a profound loss of pigmented cells in the skin eye and inner ear results, as well as osteopetrosis and defects in natural killer and mast cells. These melanocyte isoforms have been shown by two dimensional tryptic mapping to differ in c-Kit-induced phosphorylation. Osteopetrotic rat strain harbors a large genomic deletion encompassing the 3
Synonyms	Microphthalmia-associated transcription factor, Mi-protein
Reference Data

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