Macrophages Mouse Monoclonal Antibody [Clone ID: BA4D5]

Specifications

Product Data
Clone Name	BA4D5
Applications	FC
Recommend Dilution	Flow Cytometry: Use 10 µl of neat antibody to label 1x106 cells in 100 µl.  Membrane permeabilisation is required.
Reactivity	Porcine
Host	Mouse
Clonality	Monoclonal
Immunogen	Porcine alveolar macrophages. Spleen cells from immunised BALB/c mice were fused with cells of the mouse SP2/0 mouse myeloma cell line.
Specificity	This antibody recognises Porcine cells of the monocyte/macrophage lineage. Expression of the antigen is increased with maturation, with higher expression on peritoneal and alveolar macrophages. Some expression has also been observed on peripheral blood lymphocytes. The antigen recognised by this antibody Cat.-No AM05890FC-N (clone BA4D5) has a broad tissue distribution. Clone BA4D5 stains macrophages in a range of tissues, including the thymus, spleen periarteriolar lymphoid sheath (PALS), spleen red pulp and the Peyer's patches. It is believed that clone BA4D5 may be specific for porcine CD68, although the protein recognised by this antibody has not yet been fully characterised. The protein is expressed on the cell surface, although it is most abundantly expressed in the cytoplasm.
Formulation	PBS, pH 7.4 Label: FITC State: Liquid purified IgG fraction Stabilizer: 1% BSA Preservative: 0.09% Sodium Azide Label: Fluorescein Isothiocyanate Isomer 1
Concentration	lot specific
Purification	Affinity Chromatography on Protein G
Conjugation	FITC
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing.
Synonyms	Macrophage marker
Reference Data

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