GFP Mouse Monoclonal Antibody [Clone ID: N/K]

Specifications

Product Data
Clone Name	N/K
Applications	ELISA, IHC, WB
Recommend Dilution	ELISA: 1/1000-1/2000.  Western blot: 1/2000-1/5000. Immunohistochemistry on Frozen Sections: 1/500-1/1000. Immunohistochemistry on Paraffin Sections: 1/500-1/1000.
Reactivity	GFP
Host	Mouse
Clonality	Monoclonal
Immunogen	GFP-BSA conjugate
Specificity	This antibody recognises various forms of Green Fluorescent Protein including eGFP and those forms found in vectors supplied by Clontech and Invitrogen. The antibody does not cross react with RFP. Cross reactivity with YFP has not been tested.
Formulation	Phosphate buffered saline State: Purified State: Liquid purified IgG fraction Stabilizer: BSA
Conjugation	Unconjugated
Storage Condition	Upon receipt, store undiluted (in aliquots) at -20°C. Avoid repeated freezing and thawing.
Database Link	UniProt ID P42212
Background	Green fluorescence protein (GFP) is a 27 kDa protein derived from the jellyfish Aequorea victoria, which emits green light (emission peak at a wavelenth of 509 nm) when excited by blue light (excitation peak at a wavelenth of 395 nm). Green Fluorescent Protein (GFP) has become an invaluable tool in cell biology research, since its intrinsic fluorescence can be visualized in living cells. GFP fluorescence is stable under fixation conditions and suitable for a variety of applications. GFP has been widely used as a reporter for gene expression, enabling researchers to visualize and localize GFP-tagged proteins within living cells without the need for chemical staining. Other applications of GFP include assessment of protein protein interactions through the yeast two hybrid system and measurement of distance between proteins through fluorescence energy transfer (FRET) protocols. GFP technnology has considerably contributed to a greater understanding of cellular physiology.
Synonyms	Green fluorescent protein, GFP-Tag
Reference Data

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