Cd22 Rat Monoclonal Antibody [Clone ID: 2D6]

Specifications

Product Data
Clone Name	2D6
Applications	FC, FN, IP
Recommend Dilution	Flow Cytometry (Ref.2). Functional Assay: Blocking of B cell homotypic adhesion (Ref.2). Immunoprecipitation: (Ref.2)
Reactivity	Mouse
Host	Rat
Clonality	Monoclonal
Specificity	This antibody is specific to CD22 (Mr 150 kDa), a B Lymphocyte Adhesion Molecule.
Formulation	100 mM Borate Buffered Saline, pH 8.2. No preservatives or amine-containing buffer salts added. State: Purified State: Liquid purified Ig fraction
Concentration	lot specific
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	CD22 antigen
Database Link	Entrez Gene 12483 Mouse UniProt ID P35329
Background	CD22, also known as Lyb-8, is a heterodimeric transmembrane glycoprotein and a member of a structurally related group of immunoglobulin (Ig) superfamily domain-containing proteins called the sialoadhesin family. It is detected in the cytoplasm early in B cell development (late pro-B cell stage), appears on the cell surface concomitant with IgD, and is found on most IgM+IgD+ mature B lymphocytes. Expression is lost with terminal differentiation of B cells and is absent on plasma cells. Activation of B cells via cross-linking of surface Ig increases CD22 expression. (Ref.1,2) CD22 associates with the B cell receptor (BCR) complex and mediates intercellular adhesion (Ref.2-4). Its intracellular domain is phosphorylated after antigen receptor crosslinking and is involved in negative regulation of B-cell activation (Ref.6-9).
Synonyms	SIGLEC2, Siglec-2, B-cell receptor CD22, Leu-14, BL-CAM
Reference Data

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