CD49e / ITGA5 Rat Monoclonal Antibody [Clone ID: 5H10-27]

Specifications

Product Data
Clone Name	5H10-27
Applications	FC, FN, IHC
Recommend Dilution	Flow Cytometry. (Ref.1) Immunohistochemistry on Frozen Sections: (Ref.7) Functional Assays: In vitro blocking of adhesion. (Ref.1,2-4,6) T-cell co-stimulation. (Ref.3)
Reactivity	Mouse
Host	Rat
Clonality	Monoclonal
Immunogen	C57BL/6 x A/J F1 mouse mast cell line MC/9. (Ref.1)
Specificity	This antibody is specific to alpha-5 subunit of the integrin alpha-5 beta-1 Fibronectin Receptor. It reacts with the alpha-5 chain (VLA-5) of the CD49e/CD29 heterodimeric Fibronectin Receptor. (Ref.1-5) Soluble 5H10-27 antibody inhibits VLA-5-mediated functions in vitro. (Ref.1,2-4,6) Immobilized 5H10-27 has also been demonstrated to co-stimulate the proliferative response of CD8+ T cells to plate-bound anti-CD3 monoclonal antibody. (Ref.3)
Formulation	PBS containing no preservatives. State: Low Endotoxin State: Liquid purified Ig fraction.
Concentration	lot specific
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted (in aliquots) at -20°C. Avoid repeated freezing and thawing.
Gene Name	integrin alpha 5 (fibronectin receptor alpha)
Database Link	Entrez Gene 16402 Mouse UniProt ID P11688
Background	Integrin alpha 5 belongs to the integrin alpha chain family. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. This gene encodes the integrin alpha 5 chain. Alpha chain 5 undergoes post translational cleavage in the extracellular domain to yield disulfide linked light and heavy chains that join with beta 1 to form a fibronectin receptor. In addition to adhesion, integrins are known to participate in cell surface mediated signalling. It is expressed on thymocytes, activated T cells, mast cells, and a variety of mouse cell lines.
Synonyms	Integrin alpha-5, Fibronectin receptor subunit alpha, Integrin alpha-F, VLA-5, VLA5, FNRA
Reference Data

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