H2-D1 Mouse Monoclonal Antibody [Clone ID: 28-14-8]

Specifications

Product Data
Clone Name	28-14-8
Applications	CT, FC, FN, IHC, IP
Recommend Dilution	Flow Cytometry: ≤ 1 µg/106 cells. (Ref.4,7,8). Immunoprecipitation (Ref.2,3). Complement-mediated cytotoxicity (Ref.1,5,6). Immunohistochemistry on Frozen Sections. (Ref.4). Blocking (Ref.6).
Reactivity	Mouse
Host	Mouse
Clonality	Monoclonal
Immunogen	C3H.SW mouse splenocytes.
Specificity	This antibody is specific to an epitope in the α3 domain of H-2Db in the presence or absence of ß2 microglobulin. It cross reacts with the α3 domain of H-2Ld, but not Kd or Dd, and with H-2Dq and/or Lq. The antibody has been shown to block H-2Ld-specific and H-2Ld-restricted antigen-specific lysis of target cells by cytotoxic T lymphocytes but it does not block recognition of H-2Ld-positive target cells by Ly-6G2-positive NK cells. Customer feedback: Clone 28-14-8 does not cross-react with Human cells.
Formulation	1.0 ml Borate Buffered Saline, pH 8.2. No preservatives or amine-containing buffer salts added. State: Purified State: Liquid purified Ig fraction.
Concentration	lot specific
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted at 2-8°C.
Gene Name	histocompatibility 2, D region locus 1
Database Link	Entrez Gene 14964 Mouse UniProt ID P01899
Background	The “classical” MHC Class I molecules are histocompatibility antigens encoded by the H-2 gene complex and consist of heterodimers of highly polymorphic α chains noncovalently associated with the invariant ß2-microglobulin. These antigens are expressed on most nucleated cells but expression varies on different cell types. MHC Class I molecules present endogenously synthesized peptides to CD8+ T lymphocytes which are usually cytotoxic T cells. MHC Class I antigens expressed on thymic epithelial cells regulate the positive and negative selection of CD8+ T cells during T cell ontogeny.
Synonyms	H-2D(B), H2-D1
Reference Data

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