GM CSF (CSF2) Rat Monoclonal Antibody [Clone ID: BVD2-21C11]

Specifications

Product Data
Clone Name	BVD2-21C11
Applications	ELISA, FC, IF
Recommend Dilution	ELISA Detection: Biotinylated Monoclonal antibody AM08177BT-N is the preferred detection antibody in a Sandwich ELISA for quantifying human GM-CSF protein levels. (Ref.1-4). It should be paired with purified with purified recombinant human GM-CSF as the standard. Immunofluorescence/Flow Cytometry: This antibody is useful for Intracytoplasmic staining and Flow Cytometric analysis to identify and enumerate GM-CSF-positive cells within mixed cell populations. Recombinant human GM-CSF (Cat#AM08177PU-N should be used as a Specificity Control.
Reactivity	Human
Host	Rat
Clonality	Monoclonal
Immunogen	E. coli-expressed human GM-CSF.
Specificity	This antibody is specific to Human Granulocyte/Macrophage–Colony Stimulating Factor (GM-CSF)1-4. The BVD2-21C11 antibody has been reported to cross react with GM-CSF from Rhesus monkey. (This is a Neutralizing antibody).
Formulation	PBS containing 0.09% Sodium Azide as preservative. Label: Biotin State: Liquid purified Ig fraction.
Concentration	lot specific
Conjugation	Biotin
Storage Condition	Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	colony stimulating factor 2
Database Link	Entrez Gene 1437 Human UniProt ID P04141
Background	GM-CSF is a hematopoietic growth factor that stimulates the development of neutrophils and macrophages and promotes the proliferation and development of early erythroid megakaryocytic and eosinophilic progenitor cells. It is produced in by endothelial cells, monocytes, fibroblasts and T-lymphocytes. GM-CSF inhibits neutrophil migration and enhances the functional activity of the mature end-cells. The human and murine molecules are species-specific and exhibit no cross-species reactivity.
Synonyms	CSF2, GMCSF, Sargramostim, Molgramostin
Reference Data

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