TLR4 Mouse Monoclonal Antibody [Clone ID: 1E11]

Specifications

Product Data
Clone Name	1E11
Applications	ELISA, FC
Recommend Dilution	ELISA: 1/2000-1/4,000. Flow Cytometry: ≤ 3μg/106 cells.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Specificity	This antibody is specific for Toll-like Receptor 4 (TLR4).
Formulation	100 mM Borate buffered saline, pH 8.2. No preservatives or amine-containing buffer salts added. State: Purified State: Liquid purified IgG fraction
Concentration	lot specific
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	toll like receptor 4
Database Link	Entrez Gene 7099 Human UniProt ID O00206
Background	The Toll-like receptor (TLR) family is comprised of Type I transmembrane proteins characterized by an extracellular leucine-rich domain and a cytoplasmic tail that contains a conserved region call the Toll/IL-1 (TIR) domain. First discovered in Drosophila, TLRs recognize specific molecular patterns that are present in microbial components and respond to these components in order eliminate or limit invading microbes. To date, ten mammalian homologs of TLRs have been described. TLR4 is involved in the recognition of lipopolysaccharide (LPS), an abundant glycolipid of Gram-negative bacteria. Through its association with MD-2 and CD14, TLR4 recognizes and initiates multiple intracellular signaling events such as the activation of NF-kB leading to the ultimate synthesis and release of a number of proinflammatory mediators including interleukin-1 (IL-1), interleukin-6 (IL-6), interleukin-8 (IL-8) and tumor necrosis factor-α (TNF-alpha). TLR4 has also been associated in the recognition of and lipocheic acid (LTA), Hsp60 and Hsp70 (Ref.1-10)
Synonyms	Toll-like receptor 4
Reference Data
Protein Families	Druggable Genome, Transmembrane
Protein Pathways	Pathogenic Escherichia coli infection, Toll-like receptor signaling pathway

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