Perforin (PRF1) (C-term) Mouse Monoclonal Antibody [Clone ID: 5B10]

Specifications

Product Data
Clone Name	5B10
Applications	IHC
Recommend Dilution	Immunohistochemistry on Paraffin Embedded Sections: 1/10-1/20 in an ABC method (60 minutes at room temperature). Paraffin-embedded tissue sections require high temperature antigen unmasking using 10 mM citrate buffer, pH 6.0 prior to immunostaining. Positive Control: Spleen.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Recombinant protein corresponding to an external region of the C-terminus of the perforin molecule.
Specificity	This antibody is specific to a 70 kD protein, which is a potent cytolytic pore-forming protein. Cellular Localization: Cytoplasmic.
Formulation	State: Supernatant State: Tissue Culture Supernatant containing Sodium Azide as a preservative.
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted at 2-8°C.
Gene Name	perforin 1
Database Link	Entrez Gene 5551 Human UniProt ID P14222
Background	Perforin is one of the major cytolytic proteins of cytolytic granules. One of the main pathways of lymphocyte mediated cytolysis entails the secretion onto target membranes of cytolytic granules contained in cytolytic effector lymphocytes of T cell or NK cell type. Perforin is a cytolytic mediator and is stored in and released by cytoplasmic granules. Perforin is involved in the killing mediated by cytotoxic lymphocytes, which represents an important mechanism in the immune defense against tumors and virus infections. Human perforin is a 555 amino acid protein with a 21 amino acid signal peptide. It has a molecular weight of 70 to 75 kDa. Perforin is a pore forming protein with a mechanism of transmembrane channel formation similar to C9 and homology between perforin and C9 has been demonstrated at their respective functionally conserved regions. Studies show that perforin is expressed only in killer cell lines and not in helper T lymphocytes or other tumor cells tested.
Synonyms	Perforin-1, Pore forming protein, PFN, PFP, Cytolysin
Reference Data

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.