CD45 (PTPRC) Mouse Monoclonal Antibody [Clone ID: 3G4]

Specifications

Product Data
Clone Name	3G4
Applications	ELISA, IHC, WB
Recommend Dilution	ELISA. Western blot (1/1,000-1/3,000), recommended starting dilution is 1/2000. Immunohistochemistry on Paraffin Sections (10 µg/ml). Heat induced antigen retrieval in pH 6.0 citrate buffer is recommended.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Recombinant human CD45 (1029-1249 aa) purified from E. coli.
Specificity	The antibody recognizes Human CD45. Other species not tested.
Formulation	PBS, pH 7.4 containing 0.02% Sodium Azide and 10% Glycerol State: Purified State: Liquid purified Ig fraction
Concentration	lot specific
Purification	Protein-G affinity chromatography
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C for up to two weeks or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	protein tyrosine phosphatase, receptor type C
Database Link	Entrez Gene 5788 Human UniProt ID P08575
Background	CD45 belongs to a family of heavily glycosylated, high molecular weight transmembrane glycoproteins expressed on all cells of haematopoietic origin. Structurally heterogeneous, CD45 consists of a family of eight isoforms ranging from 180,000 to 220,000 MW generated by alternative splicing of three exons differentially expressed on subpopulation of lymphocytes. CD45 protein tyrosine phosphatase activity plays a central role in T-cell activation. CD45 plays an important role in signal transduction inhibition or upregulation of various immunological functions.
Synonyms	PTPRC, Leukocyte common antigen, L-CA, T200
Reference Data

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