alpha smooth muscle Actin (ACTA2) (N-term) Mouse Monoclonal Antibody [Clone ID: ASM-1]

Specifications

Product Data
Clone Name	ASM-1
Applications	IF, IHC, WB
Recommend Dilution	Immunofluorescence Microscopy Immunoblotting (Western). Cytological Material. Immunohistochemistry on Frozen and Paraffin-Embedded Tissue. Pretreatment: With paraffin embedded sections, protease pretreatment is required prior to antibody application. Working Dilution: Ready-to-use for Immunohistochemical application. Incubation Time: 1 h at RT; extended with paraffin.
Reactivity	Bovine, Chicken, Equine, Human, Mouse, Rat
Host	Mouse
Clonality	Monoclonal
Immunogen	Synthetic N-terminus decapeptide of alpha-smooth-muscle isoform of Actin.
Specificity	ASM-1 represents an excellent marker for myogenic soft tissue tumors and smooth muscle differentiation. Polypeptide reacting: specific for alpha-smooth-muscle isoform of actin (Mr 43,000). Tumors Specificallly Detected: leiomyosarcoma, leiomyoma, certain stromal cells surrounding infiltrating ductal carcinoma of breast. Tested Reactivities on Cultured Cell lines: Stress fibers of smooth muscle-derived cells and some smooth muscle subtype fibroblasts.
Formulation	PBS, pH 7.4 State: Purified State: Liquid purified IgG fraction Stabilizer: 0.5% BSA Preservative: 0.09% Sodium Azide
Purification	Affinity Chromatography on Protein A
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluited at 2-8°C
Gene Name	actin, alpha 2, smooth muscle, aorta
Database Link	Entrez Gene 11459 MouseEntrez Gene 29437 RatEntrez Gene 59 Human UniProt ID P62736
Background	The two major cytoskeletal proteins implicated in cell motility are actin and myosin. Actin and myosin are constituents of many cell types and are involved in a myriad of cellular processes including locomotion, secretion, cytoplasmic streaming, phagocytosis and cytokinesis. Although actin is one of the most conserved eukaryotic proteins, it is expressed in mammals and birds as at least six isoforms characterized by electrophoresis and amino acid sequence analysis. Four of them represent the differentiation markers of muscle tissues and two are found in practically all cells.
Synonyms	ACTSA, ACTVS, Alpha-actin-2
Reference Data
Protein Families	Stem cell - Pluripotency

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