Cytokeratin 8 (KRT8) (+ KRT18) Mouse Monoclonal Antibody [Clone ID: CK207]

Specifications

Product Data
Clone Name	CK207
Applications	IF, IHC
Recommend Dilution	Immunohistochemistry. Immunocytochemistry (Frozen or Formalin-Fixed Paraffin-Embedded (FFPE) tissue sections and cell smears). For IHC dilute concentared antibody at 1/50-1/100, use streptavidin~biotin system or polymer system, incubate 30 minutes at room temperature. For FFPE tissue sections antigen retriever like trypsin or citrate is required. Citrate antigen retriever: Boil tissue in 10 mM citrate, pH 6.0 for 10-15 minutes followed by cooling for 10-15 minutes at RT. Biotinylated antibody can be diluted 1/40-1/80 for IHC. Recommended Positive Control: Human breast, any GI tissue or skin.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Purified keratins.
Specificity	This antibody recognizes all simple epithelia and glandular (thyroid, breast, GI tract, respiratory tract, urinary tract). Most squamous carcinomas and all adenocarcinomas are stained by this antibody and keratinizing squamous carcinomas are mostly negative. Cellular Localization: Cytoplasmic.
Formulation	PBS, pH 7.4 Label: Biotin State: Purified State: Liquid purified Ig fraction Stabilizer: 1% BSA Preservative: 0.05% Sodium Azide
Concentration	lot specific
Purification	Protein A Chromatography
Conjugation	Biotin
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	keratin 8
Database Link	Entrez Gene 3856 Human UniProt ID P05787
Background	Cytokeratins are intermediate filament keratins found in the intracytoplasmic cytoskeleton of epithelial tissue There are two types of Cytokeratins: the low weight, acidic type I cytokeratins and the high weight, basic or neutral type II. Cytokeratins are usually found in pairs comprising a type I Cytokeratin and a type II cytokeratin. The high molecular weight cytokeratins, which are the basic or neutral cytokeratins, comprise subtypes CK1, CK2, CK3, CK4, CK5, CK6, CK7, CK8 and CK9. The low molecular weight cytokeratins, which are the acidic cytokeratins, comprise subtypes CK10, CK12, CK 13, CK14, CK16, CK17, CK18, CK19 and CK20.
Synonyms	KRT8, CYK8, Cytokeratin-8, CK8, Keratin-8, K8, Cytokeratin endo A
Reference Data
Protein Families	Druggable Genome

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