acidic Cytokeratin Mouse Monoclonal Antibody [Clone ID: AE1]

Specifications

Product Data
Clone Name	AE1
Applications	IF, IHC
Recommend Dilution	Suitable for Immunohistochemistry and Immunocytochemistry (Frozen or Formalin-Fixed Paraffin-Embedded (FFPE) tissue sections and cell smears). For IHC dilute concentared antibody at 1/50-1/100, use streptavidin~biotin system or polymer system, incubate 30 minutes at room temperature. FFPE tissue section requires antigen retrieval (boiling tissue in 10 mM citrate, pH 6.0 for 10-15 minutes followed by cooling for 10-15 minutes). Recommended Positive Control: Human skin, squamous cell Ca.
Reactivity	Bovine, Chicken, Human, Monkey, Mouse, Porcine, Rabbit, Rat
Host	Mouse
Clonality	Monoclonal
Immunogen	Human epithelial keratin.
Specificity	This antibody recognizes low molecular weight cytokeratins (CK 10 (56.5), CK14 (50), CK15 (50), CK16 (48) and CK19(40) of the acidic family. Cellular Localization: Cytoplasmic.
Formulation	PBS, pH 7.4 State: Purified State: Liquid purified Ig fraction Stabilizer: 1% BSA Preservative: 0.05% Sodium Azide
Concentration	lot specific
Purification	Protein A Chromatography
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Background	Cytokeratins are intermediate filament keratins found in the intracytoplasmic cytoskeleton of epithelial tissue There are two types of Cytokeratins: the low weight, acidic type I cytokeratins and the high weight, basic or neutral type II. Cytokeratins are usually found in pairs comprising a type I Cytokeratin and a type II cytokeratin. The high molecular weight cytokeratins, which are the basic or neutral cytokeratins, comprise subtypes CK1, CK2, CK3, CK4, CK5, CK6, CK7, CK8 and CK9. The low molecular weight cytokeratins, which are the acidic cytokeratins, comprise subtypes CK10, CK12, CK 13, CK14, CK16, CK17, CK18, CK19 and CK20.
Synonyms	Cytokeratin type I, Keratin type I, LMW Cytokeratins
Reference Data

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.