PCNA Mouse Monoclonal Antibody [Clone ID: PC10]

Specifications

Product Data
Clone Name	PC10
Applications	IF, IHC, WB
Recommend Dilution	Immunohistochemistry and Immunocytochemistry Frozen or Formalin-Fixed Paraffin-Embedded (FFPE) tissue sections and cell smears). For IHC dilute conc. antibody at 1/50-1/200, use streptavidin~biotin system or polymer system, incubate 30 minutes at room temperature. FFPE tissue section requires antigen retriever (boiling tissue in 10 mM citrate, pH 6.0 for 20 mins, followed by cooling at RT for 20 mins). Recommended Positive Control: Human tonsil, lymph node. Western Blot: 1/100-1/400.
Reactivity	Human, Insect, Mouse, Rat, Yeast
Host	Mouse
Clonality	Monoclonal
Immunogen	Recombinant Rat PCNA protein.
Specificity	This antibody is specific for PCNA. PC10 reacts specifically with a 36kD nuclear protein of the Proliferating Cell Nuclear Antigen (PCNA). PCNA is an excellent marker of proliferative cells in routinely processed tissue sections. Cellular Localization: Nuclear.
Formulation	PBS, pH 7.4 State: Purified State: Liquid purified IgG fraction Stabilizer: 1% BSA Preservative: 0.05% Sodium Azide
Concentration	lot specific
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	proliferating cell nuclear antigen
Database Link	Entrez Gene 5111 Human UniProt ID P12004
Background	Proliferating Cell Nuclear Antigen, commonly known as PCNA, is a protein that acts as a processivity factor for DNA polymerase delta in eukaryotic cells. The protein encoded by this gene is found in the nucleus and is a cofactor of DNA polymerase delta. The encoded protein acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication. In response to DNA damage, this protein is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway. Two transcript variants encoding the same protein have been found for this gene. Pseudogenes of this gene have been described on chromosome 4 and on the X chromosome. PCNA was originally identified as an antigen that is expressed in the nuclei of cells during the DNA synthesis phase of the cell cycle It is increased during late G1 phase and S phase of the cell cycle and declines during G2 and M phases.
Synonyms	Cyclin
Reference Data

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