MITF Mouse Monoclonal Antibody [Clone ID: C5+D5]

Specifications

Product Data
Clone Name	C5+D5
Applications	IHC
Recommend Dilution	Immunohistochemistry on Formalin-Fixed, Paraffin-Embedded Sections: 1/50-1/200 Pretreatment of deperaffinized tissue with heat-induced epitope retrieval is recommended. Use Polymer anti Mouse/Rabbit IgG as a detection system. Positive Control: Melanoma.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	N-terminal fragment of Human Mi protein.
Specificity	Clone D5 cocktail is especially designed for sensitive detection of Mi protein. it reacts with both melanocytic and non-melanocytic isoforms of Mi. Cellular Localization: Cytoplasmic.
Formulation	State: Supernatant State: Liquid Tissue Culture Supernatant Stabilizer: 0.2% BSA Preservative: 15mM Sodium Azide
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted at 2-8°C.
Gene Name	melanogenesis associated transcription factor
Database Link	Entrez Gene 4286 Human UniProt ID O75030
Background	Mi is a basic helix-loop-helix-leucin zipper (b-HLH-ZIP) transcription factor implicated in pigmentation, mast cells and bone development. The mutation of Mi causes Waardenburg Syndrome type II in humans. In mice, a profound loss of pigmented cells in the skin eye and inner ear results, as well as osteopetrosis and defects in natural killer and mast cells. There are two known isoforms of Mi differing by 66 amino acids at the NH2 terminus. Shorter forms are expressed in melanocytes and run as two bands at 52kDa and 56kDa, while the longer Mi form runs as a cluster of bands at 60-70kDa in osteoclasts and in B16 melanoma cells (but not other melanoma cell lines), as well as mast cells and heart.
Synonyms	Microphthalmia-associated transcription factor, Mi-protein
Reference Data
Protein Families	Druggable Genome, Transcription Factors
Protein Pathways	Melanogenesis, Melanoma, Pathways in cancer

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