CD28 Mouse Monoclonal Antibody [Clone ID: CD28.2]

Specifications

Product Data
Clone Name	CD28.2
Applications	FC, FN, IF, IHC, IP, WB
Recommend Dilution	Flow cytometry. Immunoprecipitation. Western blot. Immunohistochemistry (frozen sections). Immunocytochemistry. Functional application: T cell costimulation.
Reactivity	Human, Primate
Host	Mouse
Clonality	Monoclonal
Immunogen	DC28.1.3.3 murine T cell hybridoma transfected with human CD28 cDNA
Specificity	This antibody reacts with CD28, a disulfide-linked homodimeric type I glycoprotein (monomer of Mw 44 kDa) which is a critical costimulatory receptor of T cells.
Formulation	Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4 State: Purified State: Liquid Ig fraction
Concentration	lot specific
Purification	Protein A affinity chromatography; purity > 95% (by SDS-PAGE)
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C. DO NOT FREEZE!
Gene Name	CD28 molecule
Database Link	Entrez Gene 940 Human UniProt ID P10747
Background	CD28 is the critical T cell costimulatory receptor which provides to the cell the important second activation signal by binding CD80 and CD86 that are expressed by antigen presenting cells. Besides its costimulation role CD28 functions in preventing T cells from anergic hyporesponsive state or from undergoing premature apoptotic cell death. CD28 is also expressed on human fetal NK cells and some NK cell lines, whereas on murine NK cells the CD28 expression is much broader.
Synonyms	TP44
Reference Data
Protein Families	Druggable Genome, Transmembrane
Protein Pathways	Allograft rejection, Autoimmune thyroid disease, Cell adhesion molecules (CAMs), Graft-versus-host disease, Systemic lupus erythematosus, T cell receptor signaling pathway, Type I diabetes mellitus, Viral myocarditis

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