c-Myc (MYC) Mouse Monoclonal Antibody [Clone ID: Myc.A7]

Specifications

Product Data
Clone Name	Myc.A7
Applications	IF, IP, WB
Recommend Dilution	Western blot: 1/1,0001/4,000. Immunoprecipitation. Immunocytochemistry/Immunofluorescence: 1/200-1/400.
Reactivity	Mammalian
Host	Mouse
Clonality	Monoclonal
Immunogen	A synthetic peptide KLH conjugated (EQKLISEEDL) corresponding to amino acids 410-419 of transcription factor c-Myc.
Specificity	Myc-tag Monoclonal Antibody recognises over-expressed proteins containing Myc epitope tag fused to either amino- or carboxy-termini of targeted proteins in transfected mammalian cells. Reacts with Human, Mouse, Rat and others.
Formulation	PBS, pH 7.4 State: Aff - Purified State: Liquid purified IgG fraction Preservative: 0.05% Sodium Azide
Concentration	lot specific
Purification	Affinity Chromatography
Conjugation	Unconjugated
Storage Condition	Upon receipt, store undiluted (in aliquots) at -20°C. Avoid repeated freezing and thawing.
Gene Name	v-myc avian myelocytomatosis viral oncogene homolog
Database Link	Entrez Gene 4609 Human UniProt ID P01106
Background	Epitope tags are short peptide sequences that are easily recognized by tag-specific antibodies. Due to their small size, epitope tags do not affect the tagged protein’s biochemical properties. Most often sequences encoding the epitope tag are included with target DNA at the time of cloning to produce fusion proteins containing the epitope tag sequence. This allows anti-epitope tag antibodies to serve as universal detection reagents for any tag containing protein produced by recombinant means. This means that anti-epitope tag antibodies are a useful alternative to generating specific antibodies to identify, immunoprecipitate or immunoaffinity purify a recombinant protein. The anti-epitope tag antibody is usually functional in a variety of antibody-dependent experimental procedures. Expression vectors producing epitope tag fusion proteins are available for a variety of host expression systems including bacteria, yeast, insect and mammalian cells.
Synonyms	myc tag, myc-tag, c-myc tag
Reference Data
Protein Families	Druggable Genome, Embryonic stem cells, Induced pluripotent stem cells, Stem cell - Pluripotency, Stem cell relevant signaling - JAK/STAT signaling pathway, Stem cell relevant signaling - TGFb/BMP signaling pathway, Stem cell relevant signaling - Wnt Signaling pathway, Transcription Factors
Protein Pathways	Acute myeloid leukemia, Bladder cancer, Cell cycle, Chronic myeloid leukemia, Colorectal cancer, Endometrial cancer, ErbB signaling pathway, Jak-STAT signaling pathway, MAPK signaling pathway, Pathways in cancer, Small cell lung cancer, TGF-beta signaling pathway, Thyroid cancer, Wnt signaling pathway

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