HA Epitope Tag (YPYDVPDYA) Mouse Monoclonal Antibody [Clone ID: 26D11]

Specifications

Product Data
Clone Name	26D11
Applications	ELISA, IF, IP, WB
Recommend Dilution	ELISA: 1/2000. Western blot: 1/5000. Incubate membrane with diluted antibody in 5% w/v nonfat dry milk, 1 TBS, 0.05% Tween-20 at 4°C with gentle shaking, overnight. Immunoprecipitation: 1/100. Immunofluorescence: 1/1000.
Reactivity	All Species, Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Synthetic peptide containing the Infuenza Hemagglutinin epitope (YPYDVPDYA) (KLH-coupled).
Specificity	This antibody detects over-expressed proteins containing the HA epitope tag.
Formulation	State: Aff - Purified State: Liquid purified Ig fraction
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Background	Plasmid vectors for the expression of coding regions of eukaryotic genes in bacterial, insect and mammalian hosts are in common usage; such expression vectors are frequently used to encode hybrid fusion proteins consisting of a eukaryotic target protein and a specialized region designed to aid in the purification and visualization of the target protein. For example, the pCDM8 expression vector and derivatives thereof encode fusions between the target protein and an 11 amino acid peptide derived from the influenza protein hemagglutinin (HA). The HA epitope tag is useful in Western blotting and immunohistochemical localization of expressed fusion proteins when examined with antibodies raised specifically against the HA-epitope tag.
Synonyms	Hemagglutinin Tag, HA-Tag, HA Tag
Note	Use an anti-Mouse secondary antibody to detect the 26D11 antibody.
Reference Data

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